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Journal of Virology, November 2001, p. 11079-11087, Vol. 75, No. 22
Department of Pediatrics, Division of
Infectious Diseases,1 and Department of
Pathology,2 Yale University School of
Medicine, New Haven, Connecticut 06520
Received 29 May 2001/Accepted 16 August 2001
Foreign glycoproteins expressed in recombinant
vesicular stomatitis virus (VSV) can elicit specific and protective
immunity in the mouse model. We have previously demonstrated the
expression of respiratory syncytial virus (RSV) G (attachment) and F
(fusion) glycoprotein genes in recombinant VSV. In this
study, we demonstrate the expression of RSV F and G
glycoproteins in attenuated, nonpropagating VSVs which lack
the VSV G gene (VSV
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.22.11079-11087.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Replication-Competent or Attenuated, Nonpropagating Vesicular
Stomatitis Viruses Expressing Respiratory Syncytial Virus (RSV)
Antigens Protect Mice against RSV Challenge
G) and the incorporation of these RSV proteins
into recombinant virions. We also show that intranasal vaccination of
mice with nondefective VSV recombinants expressing RSV G (VSV-RSV G) or
RSV F (VSV-RSV F) elicited RSV-specific antibodies in serum (by
enzyme-linked immunosorbent assay [ELISA]) as well as neutralizing
antibodies to RSV and afford complete protection against RSV challenge.
In contrast, VSV
G-RSV F induced detectable serum antibodies to RSV
by ELISA, but no detectable neutralizing antibodies, yet it still
protected from RSV challenge. VSV
G-RSV G failed to induce any
detectable serum (by ELISA) or neutralizing antibodies and failed to
protect from RSV challenge. The attenuated, nonpropagating VSV
G-RSV
F is a particularly attractive candidate for a live attenuated
recombinant RSV vaccine.
*
Corresponding author. Mailing address: Department of
Pediatrics, Division of Infectious Diseases, LSOG 416, Yale University School of Medicine, P.O. Box 208064, New Haven, CT 06520-8064. Phone:
(203) 785-6778. Fax: (203) 785-6961. E-mail:
jeffrey.kahn{at}yale.edu.
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