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Journal of Virology, November 2001, p. 11079-11087, Vol. 75, No. 22
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.22.11079-11087.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Replication-Competent or Attenuated, Nonpropagating Vesicular Stomatitis Viruses Expressing Respiratory Syncytial Virus (RSV) Antigens Protect Mice against RSV Challenge

Jeffrey S. Kahn,1,* Anjeanette Roberts,2 Carla Weibel,1 Linda Buonocore,2 and John K. Rose2

Department of Pediatrics, Division of Infectious Diseases,1 and Department of Pathology,2 Yale University School of Medicine, New Haven, Connecticut 06520

Received 29 May 2001/Accepted 16 August 2001

Foreign glycoproteins expressed in recombinant vesicular stomatitis virus (VSV) can elicit specific and protective immunity in the mouse model. We have previously demonstrated the expression of respiratory syncytial virus (RSV) G (attachment) and F (fusion) glycoprotein genes in recombinant VSV. In this study, we demonstrate the expression of RSV F and G glycoproteins in attenuated, nonpropagating VSVs which lack the VSV G gene (VSVDelta G) and the incorporation of these RSV proteins into recombinant virions. We also show that intranasal vaccination of mice with nondefective VSV recombinants expressing RSV G (VSV-RSV G) or RSV F (VSV-RSV F) elicited RSV-specific antibodies in serum (by enzyme-linked immunosorbent assay [ELISA]) as well as neutralizing antibodies to RSV and afford complete protection against RSV challenge. In contrast, VSVDelta G-RSV F induced detectable serum antibodies to RSV by ELISA, but no detectable neutralizing antibodies, yet it still protected from RSV challenge. VSVDelta G-RSV G failed to induce any detectable serum (by ELISA) or neutralizing antibodies and failed to protect from RSV challenge. The attenuated, nonpropagating VSVDelta G-RSV F is a particularly attractive candidate for a live attenuated recombinant RSV vaccine.

* Corresponding author. Mailing address: Department of Pediatrics, Division of Infectious Diseases, LSOG 416, Yale University School of Medicine, P.O. Box 208064, New Haven, CT 06520-8064. Phone: (203) 785-6778. Fax: (203) 785-6961. E-mail: jeffrey.kahn{at}yale.edu.

Journal of Virology, November 2001, p. 11079-11087, Vol. 75, No. 22
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.22.11079-11087.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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