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Journal of Virology, November 2001, p. 10870-10879, Vol. 75, No. 22
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.22.10870-10879.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Role of Immunoglobulin A in Protection against Reovirus Entry into Murine Peyer's Patches†

Katherine J. Silvey, Amy B. Hutchings, Michael Vajdy,Dagger Mary M. Petzke, and Marian R. Neutra*

GI Cell Biology Laboratory, Children's Hospital, and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115

Received 27 March 2001/Accepted 11 August 2001

Reovirus type 1 Lang (T1L) infects the mouse intestinal mucosa by adhering specifically to epithelial M cells and exploiting M-cell transport to enter the Peyer's patches. Oral inoculation of adult mice has been shown to elicit cellular and humoral immune responses that clear the infection within 10 days. This study was designed to determine whether adult mice that have cleared a primary infection are protected against viral entry upon oral rechallenge and, if so, whether antireovirus secretory immunoglobulin A (S-IgA) is a necessary component of protection. Adult BALB/c mice that were orally inoculated on day 0 with reovirus T1L produced antiviral S-IgA in feces and IgG in serum directed primarily against the reovirus sigma 1 attachment protein. Eight hours after oral reovirus challenge on day 21, the Peyer's patches of previously exposed mice contained no detectable virus whereas Peyer's patches of naive controls contained up to 2,300 PFU of reovirus/mg of tissue. Orally inoculated IgA knockout (IgA-/-) mice cleared the initial infection as effectively as wild-type mice and produced higher levels of reovirus-specific serum IgG and secretory IgM than C57BL/6 wild-type mice. When IgA-/- mice were rechallenged on day 21, however, their Peyer's patches became infected. These results indicate that intestinal S-IgA is an essential component of immune protection against reovirus entry into Peyer's patch mucosa.

* Corresponding author. Mailing address: GI Cell Biology Laboratory, Enders 1220, Children's Hospital, 300 Longwood Ave., Boston, MA 02115. Phone: (617) 355-6229. Fax: (617) 264-2876. E-mail: marian.neutra{at}tch.harvard.edu.

dagger This study is dedicated to the memory of our friend and colleague Bernard N. Fields.

Dagger Present address: Chiron Corporation, Emeryville, CA 94608

Journal of Virology, November 2001, p. 10870-10879, Vol. 75, No. 22
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.22.10870-10879.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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