Selective Loss of Natural Killer T Cells by Apoptosis following Infection with Lymphocytic Choriomeningitis Virus

doi:10.1128/JVI.75.22.10746-10754.2001

This Article

	Full Text
	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Reprints and Permissions
	Copyright Information
	Books from ASM Press
	MicrobeWorld

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Hobbs, J. A.
	Articles by Brutkiewicz, R. R.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Hobbs, J. A.
	Articles by Brutkiewicz, R. R.

Previous Article | Next Article

Journal of Virology, November 2001, p. 10746-10754, Vol. 75, No. 22
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.22.10746-10754.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Selective Loss of Natural Killer T Cells by Apoptosis following Infection with Lymphocytic Choriomeningitis Virus

Jacqueline A. Hobbs,¹ Sungyoo Cho,¹ Tonya J. Roberts,¹ Venkataraman Sriram,¹ Jianhua Zhang,² Ming Xu,² and Randy R. Brutkiewicz¹^,^*

Department of Microbiology and Immunology and The Walther Oncology Center, The Walther Cancer Institute, Indiana University School of Medicine, Indianapolis, Indiana 46202,¹ and Department of Cell Biology, Neurobiology, and Anatomy, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267²

Received 20 April 2001/Accepted 11 August 2001

Natural killer T (NKT) cells, a unique subpopulation of T cells, coexpress markers also present on NK cells and recognizethe major histocompatibility complex class I-like CD1d1 molecule.We studied the effect of an acute virus infection on NKT cells.Mice were infected with the nonhepatotropic Armstrong strain oflymphocytic choriomeningitis virus (LCMV), and at various timespostinfection, mononuclear cells from the liver, peritoneum, andspleen were isolated. It was found that within 2 to 3 days, therewas a selective loss of NKT cells from the liver with an apparentrapid recovery within 8 to 14 days. There was no increase in peritonealor splenic NKT cells, indicating that NKT cells did not trafficto these tissues. This loss of NKT cells was independent of gammainterferon (IFN- $gamma$ ) and interleukin 12 (IL-12) production, butdid occur in mice treated with poly(I-C), a classical inducerof IFN- $alpha$ / $beta$ . The reduction in NKT cells was CD28 and fas/fasL independentand occurred via apoptosis. It was not observed in LCMV-infectedDNA fragmentation factor 45-deficient mice, and an increase inactive caspase 3-specific staining was found in liver NKT cellsfrom LCMV-infected and poly(I-C)-treated mice compared to uninfectedwild-type mice. Interestingly, it was also found that liver NKTcells from LCMV-infected mice were themselves infected. Theseresults suggest that the loss of NKT cells following an acuteLCMV infection could be due to the induction of IFN- $alpha$ / $beta$ resultingin NKT-cell apoptosis and is important for the host's immune responsetoLCMV.

^* Corresponding author. Mailing address: Department of Microbiology and Immunology, Indiana University School of Medicine, BuildingR4, Rm. 302, 1044 W. Walnut St., Indianapolis, IN 46202-5254.Phone: (317) 274-7589. Fax: (317) 274-7592. E-mail: rbrutkie{at}iupui.edu.

This article has been cited by other articles:

Guy, C. S., Mulrooney-Cousins, P. M., Churchill, N. D., Michalak, T. I. (2008). Intrahepatic Expression of Genes Affiliated with Innate and Adaptive Immune Responses Immediately after Invasion and during Acute Infection with Woodchuck Hepadnavirus. J. Virol. 82: 8579-8591 [Abstract] [Full Text]
Chiba, A., Dascher, C. C., Besra, G. S., Brenner, M. B. (2008). Rapid NKT Cell Responses Are Self-Terminating during the Course of Microbial Infection. J. Immunol. 181: 2292-2302 [Abstract] [Full Text]
Renukaradhya, G. J., Khan, M. A., Vieira, M., Du, W., Gervay-Hague, J., Brutkiewicz, R. R. (2008). Type I NKT cells protect (and type II NKT cells suppress) the host's innate antitumor immune response to a B-cell lymphoma. Blood 111: 5637-5645 [Abstract] [Full Text]
Pedra, J. H. F., Mattner, J., Tao, J., Kerfoot, S. M., Davis, R. J., Flavell, R. A., Askenase, P. W., Yin, Z., Fikrig, E. (2008). c-Jun NH2-Terminal Kinase 2 Inhibits Gamma Interferon Production during Anaplasma phagocytophilum Infection. Infect. Immun. 76: 308-316 [Abstract] [Full Text]
Ajuebor, M. N. (2007). Role of NKT Cells in the Digestive System. I. Invariant NKT cells and liver diseases: is there strength in numbers?. Am. J. Physiol. Gastrointest. Liver Physiol. 293: G651-G656 [Abstract] [Full Text]
Ilyinskii, P. O., Wang, R., Balk, S. P., Exley, M. A. (2006). CD1d Mediates T-Cell-Dependent Resistance to Secondary Infection with Encephalomyocarditis Virus (EMCV) In Vitro and Immune Response to EMCV Infection In Vivo. J. Virol. 80: 7146-7158 [Abstract] [Full Text]
Larkin, J., Renukaradhya, G. J., Sriram, V., Du, W., Gervay-Hague, J., Brutkiewicz, R. R. (2006). CD44 Differentially Activates Mouse NK T Cells and Conventional T Cells. J. Immunol. 177: 268-279 [Abstract] [Full Text]
Renukaradhya, G. J., Webb, T. J. R., Khan, M. A., Lin, Y. L., Du, W., Gervay-Hague, J., Brutkiewicz, R. R. (2005). Virus-Induced Inhibition of CD1d1-Mediated Antigen Presentation: Reciprocal Regulation by p38 and ERK. J. Immunol. 175: 4301-4308 [Abstract] [Full Text]
Wesche, D. E., Lomas-Neira, J. L., Perl, M., Chung, C.-S., Ayala, A. (2005). Leukocyte apoptosis and its significance in sepsis and shock. J. Leukoc. Biol. 78: 325-337 [Abstract] [Full Text]
Lin, Y., Roberts, T. J., Spence, P. M., Brutkiewicz, R. R. (2005). Reduction in CD1d expression on dendritic cells and macrophages by an acute virus infection. J. Leukoc. Biol. 77: 151-158 [Abstract] [Full Text]
Sandberg, J. K., Stoddart, C. A., Brilot, F., Jordan, K. A., Nixon, D. F. (2004). Development of innate CD4+ {alpha}-chain variable gene segment 24 (V{alpha}24) natural killer T cells in the early human fetal thymus is regulated by IL-7. Proc. Natl. Acad. Sci. USA 101: 7058-7063 [Abstract] [Full Text]
Roberts, T. J., Lin, Y., Spence, P. M., Van Kaer, L., Brutkiewicz, R. R. (2004). CD1d1-Dependent Control of the Magnitude of an Acute Antiviral Immune Response. J. Immunol. 172: 3454-3461 [Abstract] [Full Text]
Zhang, J., Dong, M., Li, L., Fan, Y., Pathre, P., Dong, J., Lou, D., Wells, J. M., Olivares-Villagomez, D., Van Kaer, L., Wang, X., Xu, M. (2003). Endonuclease G is required for early embryogenesis and normal apoptosis in mice. Proc. Natl. Acad. Sci. USA 100: 15782-15787 [Abstract] [Full Text]
Skold, M., Behar, S. M. (2003). Role of CD1d-Restricted NKT Cells in Microbial Immunity. Infect. Immun. 71: 5447-5455 [Full Text]
Wilson, M. T., Johansson, C., Olivares-Villagomez, D., Singh, A. K., Stanic, A. K., Wang, C.-R., Joyce, S., Wick, M. J., Van Kaer, L. (2003). The response of natural killer T cells to glycolipid antigens is characterized by surface receptor down-modulation and expansion. Proc. Natl. Acad. Sci. USA 100: 10913-10918 [Abstract] [Full Text]
Motsinger, A., Haas, D. W., Stanic, A. K., Van Kaer, L., Joyce, S., Unutmaz, D. (2002). CD1d-restricted Human Natural Killer T Cells Are Highly Susceptible to Human Immunodeficiency Virus 1 Infection. J. Exp. Med. 195: 869-879 [Abstract] [Full Text]

J. Bacteriol.	Mol. Cell. Biol.	Microbiol. Mol. Biol. Rev.

Clin. Vaccine Immunol.	ALL ASM JOURNALS