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Journal of Virology, November 2001, p. 10515-10519, Vol. 75, No. 21
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.21.10515-10519.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Emergence and Kinetics of Simian Immunodeficiency Virus-Specific
CD8+ T Cells in the Intestines of Macaques during
Primary Infection
Ronald S.
Veazey,1,2,*
Marie-Claire
Gauduin,1
Keith G.
Mansfield,1
Irene C.
Tham,1
John D.
Altman,3
Jeffrey D.
Lifson,4
Andrew A.
Lackner,1 and
R. Paul
Johnson1,5
New England Regional Primate Research Center,
Harvard Medical School, Southborough, Massachusetts
017721; Tulane Regional Primate Research
Center, Tulane University, Covington, Louisiana
704332; Emory Vaccine Center at Yerkes,
Emory University School of Medicine, Atlanta, Georgia
303223; SAIC, NCI-Frederick Cancer
Research and Development Center, Frederick, Maryland
217024; and Infectious Disease Unit and
Partners AIDS Research Center, Massachusetts General Hospital, Harvard
Medical School, Charlestown, Massachusetts 021295
Received 23 March 2001/Accepted 7 June 2001
In this report, three Mamu-A*01+ rhesus macaques were
examined to compare the emergence of simian immunodeficiency virus
(SIV)-specific CD8+ T cells in the intestines and blood in
early SIV infection using a major histocompatibility complex class I
tetramer complexed with the Gag181-189 peptide. Fourteen
days after intravenous inoculation with SIVmac251, large numbers of SIV
Gag181-189-specific CD8+ T cells were detected
in the intestinal mucosa (3.1 to 11.5% of CD3+
CD8+ lymphocytes) as well as in the blood (3.1 to 13.4%)
of all three macaques. By 21 days postinoculation, levels of
tetramer-binding cells had dropped in both the intestines and blood. At
day 63, however, levels of SIV Gag181-189-specific
CD8+ T cells in the intestines had rebounded in all three
macaques to levels that were higher (8.6 to 18.7%) than those at day
21. In contrast, percentages of tetramer-binding cells in the
peripheral blood remained comparatively stable (2.5 to 4.5%) at this
time point. In summary, SIV Gag181-189-specific
CD8+ T cells appeared in both the intestinal mucosa and
peripheral blood at a comparable rate and magnitude in primary SIV
infection. Given that the intestine is a major site of early viral
replication as well as the site where most of the total body lymphocyte
pool resides, these data indicate that it is also an early and
important site of development of antiviral immune responses.
*
Corresponding author: Present address: Tulane Regional
Primate Research Center, 18703 Three Rivers Rd., Covington, LA 70433. Phone: (504) 871-6228. Fax: (504) 893-1352. E-mail:
veazey{at}tpc.tulane.edu.
Journal of Virology, November 2001, p. 10515-10519, Vol. 75, No. 21
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.21.10515-10519.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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