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Journal of Virology, November 2001, p. 10493-10497, Vol. 75, No. 21
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.21.10493-10497.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Positively Charged Termini of the L2 Minor Capsid Protein Are Necessary for Papillomavirus Infection

Richard B. S. Roden,1,2,* Patricia M. Day,2 Brian K. Bronzo,1 William H. Yutzy IV,1 Yanqin Yang,1 Douglas R. Lowy,2 and John T. Schiller2

Department of Pathology, The Johns Hopkins University, Baltimore, Maryland 21205-2196,1 and Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, Maryland 20892-40402

Received 6 June 2001/Accepted 2 August 2001

Coexpression of bovine papillomavirus L1 with L2 mutants lacking either eight N-terminal or nine C-terminal amino acids that encode positively charged domains resulted in wild-type levels of viral genome encapsidation. Despite wild-type binding to the cell surface, the resulting virions were noninfectious. An L2 mutant encoding a scrambled version of the nine C-terminal residues restored infectivity, in contrast to an L2 mutant encoding a scrambled version of the N-terminal residues.

* Corresponding author. Mailing address: Department of Pathology, Room 656, Ross Research Building, The Johns Hopkins University, 720 Rutland Ave., Baltimore, MD 21205-2196. Phone: (410) 502-5161. Fax: (410) 614-3548. E-mail: roden{at}jhmi.edu.

Journal of Virology, November 2001, p. 10493-10497, Vol. 75, No. 21
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.21.10493-10497.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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