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Journal of Virology, November 2001, p. 10488-10492, Vol. 75, No. 21
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.21.10488-10492.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Epstein-Barr Virus and the Somatic Hypermutation of Immunoglobulin Genes in Burkitt's Lymphoma Cells

Reuben S. Harris,1,* Debbie S. G. Croom-Carter,2 Alan B. Rickinson,2 and Michael S. Neuberger1

MRC Laboratory of Molecular Biology, Cambridge CB2 2QH,1 and CRC Institute for Cancer Studies, University of Birmingham, Birmingham B15 2TA,2 United Kingdom

Received 21 May 2001/Accepted 25 July 2001

It has been suggested that Epstein-Barr virus (EBV) might suppress antibody maturation either by facilitating bypass of the germinal center reaction or by inhibiting hypermutation directly. However, by infecting the Burkitt's lymphoma (BL) cell line Ramos, which hypermutates constitutively and can be considered a transformed analogue of a germinal center B cell, with EBV as well as by transfecting it with selected EBV latency genes, we demonstrate that expression of EBV gene products does not lead to an inhibition of hypermutation. Moreover, we have identified two natural EBV-positive BL cell lines (ELI-BL and BL16) that hypermutate constitutively. Thus, contrary to expectations, EBV gene products do not appear to affect somatic hypermutation.

* Corresponding author. Mailing address: MRC Laboratory of Molecular Biology, Protein and Nucleic Acid Division, Hills Rd., Cambridge CB2 2QH, United Kingdom. Phone: 44 1223 402460. Fax: 44 1223 412178. E-mail: rsharris{at}mrc-lmb.cam.ac.uk.

Journal of Virology, November 2001, p. 10488-10492, Vol. 75, No. 21
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.21.10488-10492.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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