Journal of Virology, November 2001, p. 10393-10400, Vol. 75, No. 21
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.21.10393-10400.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Gene Therapy Program,1 Department of Medicine,2 Department of Pediatrics,4 and Department of Anatomy,5 Louisiana State University Health Sciences Center of New Orleans, and Bone Marrow Transplantation Program, Tulane University,3 New Orleans, Louisiana
Received 11 July 2001/Accepted 16 July 2001
We have previously reported effective gene transfer with a targeted molecular conjugate adenovirus vector through the c-kit receptor in hematopoietic progenitor cell lines. However, a c-kit-targeted recombinant retroviral vector failed to transduce cells, indicating the existence of significant differences for c-kit target gene transfer between these two viruses. Here we demonstrate that conjugation of an adenovirus to a c-kit-retargeted retrovirus vector enables retroviral transduction. This finding suggests the requirement of endosomalysis for successful c-kit-targeted gene transfer. Furthermore, we show efficient gene transfer to, and high transgene expression (66%) in, CD34-selected, c-kit+ human peripheral blood stem cells using a c-kit-targeted adenovirus vector. These findings may have important implications for future vector development in c-kit-targeted stem cell gene transfer.
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