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Journal of Virology, October 2001, p. 9991-9994, Vol. 75, No. 20
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.20.9991-9994.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Novel cis-Acting Replication Element in the Adeno-Associated Virus Type 2 Genome Is Involved in Amplification of Integrated rep-cap Sequences

Pascale Nony,1,dagger Jacques Tessier,1 Gilliane Chadeuf,1 Peter Ward,2 Aurélie Giraud,1 Marc Dugast,1 R. Michael Linden,2,3 Philippe Moullier,1 and Anna Salvetti1,*

Laboratoire de Thérapie Génique, CHU Hotel Dieu, 44035 Nantes Cedex 01, France,1 and Institute for Gene Therapy and Molecular Medecine2 and Department of Microbiology,3 Mt. Sinai School of Medicine, New York, New York 10029

Received 7 May 2001/Accepted 9 July 2001

This study identifies a region of the adeno-associated virus type 2 (AAV-2) rep gene (nucleotides 190 to 540 of wild-type AAV-2) as a cis-acting Rep-dependent element able to promote the replication of transiently transfected plasmids. This viral element is also shown to be involved in the amplification of integrated sequences in the presence of adenovirus and Rep proteins.

* Corresponding author. Mailing address: Laboratoire de Thérapie Génique, CHU Hotel Dieu, Bâtiment Jean Monnet, 30 avenue Jean Monnet, 44035 Nantes cedex 01, France. Phone: (33) 240087490. Fax: (33) 240087491. E-mail: salvetti{at}sante.univ-nantes.fr.

dagger Present address: CNRS-UMR5641, Domaine Rockefeller, 69373 Lyon Cedex O9, France.

Journal of Virology, October 2001, p. 9991-9994, Vol. 75, No. 20
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.20.9991-9994.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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