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Journal of Virology, October 2001, p. 9991-9994, Vol. 75, No. 20
Laboratoire de Thérapie Génique, CHU Hotel
Dieu, 44035 Nantes Cedex 01, France,1 and
Institute for Gene Therapy and Molecular
Medecine2 and Department of
Microbiology,3 Mt. Sinai School of Medicine, New
York, New York 10029
Received 7 May 2001/Accepted 9 July 2001
This study identifies a region of the adeno-associated
virus type 2 (AAV-2) rep gene (nucleotides 190 to 540 of
wild-type AAV-2) as a cis-acting Rep-dependent element able
to promote the replication of transiently transfected plasmids. This
viral element is also shown to be involved in the amplification of
integrated sequences in the presence of adenovirus and Rep proteins.
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.20.9991-9994.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Novel cis-Acting Replication Element in the
Adeno-Associated Virus Type 2 Genome Is Involved in Amplification
of Integrated rep-cap Sequences

*
Corresponding author. Mailing address: Laboratoire de
Thérapie Génique, CHU Hotel Dieu, Bâtiment Jean
Monnet, 30 avenue Jean Monnet, 44035 Nantes cedex 01, France. Phone:
(33) 240087490. Fax: (33) 240087491. E-mail:
salvetti{at}sante.univ-nantes.fr.
Present address: CNRS-UMR5641, Domaine Rockefeller, 69373 Lyon
Cedex O9, France.
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