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Journal of Virology, October 2001, p. 9986-9990, Vol. 75, No. 20
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.20.9986-9990.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Mapping B-Cell Epitopes of Hepatitis C Virus E2 Glycoprotein Using Human Monoclonal Antibodies from Phage Display Libraries

Francesca Bugli,1 Nicasio Mancini,1 Chang-Yuil Kang,2 Cristiana Di Campli,3 Antonella Grieco,4 Aldo Manzin,4 Armando Gabrielli,5 Antonio Gasbarrini,6 Giovanni Fadda,1 Pietro E. Varaldo,4 Massimo Clementi,7 and Roberto Burioni4,*

Istituti di Microbiologia,1 Clinica Medica3 and Patologia Medica,6 Facoltà di Medicina e Chirurgia, Università Cattolica del S. Cuore, 00168 Rome, Istituti di Microbiologia4 and Medicina Interna,5 Facoltà di Medicina e Chirurgia, Università di Ancona, 60020 Ancona, and Department of Biomedical Sciences, Università di Trieste, Via Giorgieri 22, 34100 Trieste,7 Italy, and Laboratory of Immunology, College of Pharmacy, Seoul National University, Seoul 151-742, Korea2

Received 16 April 2001/Accepted 12 July 2001

Clinical and experimental evidence indicates that the hepatitis C virus (HCV) E2 glycoprotein (HCV/E2) is the most promising candidate for the development of an effective anti-HCV vaccine. Identification of the human epitopes that are conserved among isolates and are able to elicit protective antibodies would constitute a significant step forward. This work describes the mapping of the B-cell epitopes present on the surface of HCV/E2, as recognized by the immune system during infection, by the analysis of the reciprocal interactions of a panel of human recombinant Fabs derived from an HCV-infected patient. Three unrelated epitopes recognized by antibodies with no neutralization-of-binding (NOB) activity were identified; a fourth, major epitope was defined as a clustering of minor epitopes recognized by Fabs endowed with strong NOB activity.

* Corresponding author. Mailing address: Servizio di Virologia, Istituto di Microbiologia, Facoltà di Medicina e Chirurgia, Università di Ancona, Via Conca, 60020 ANCONA, Italy. Phone: 39 071 5964849. Fax: 39 071 5964852. E-mail: r.burioni{at}libero.it.

Journal of Virology, October 2001, p. 9986-9990, Vol. 75, No. 20
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.20.9986-9990.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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