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Journal of Virology, October 2001, p. 9986-9990, Vol. 75, No. 20
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.20.9986-9990.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Mapping B-Cell Epitopes of Hepatitis C Virus E2
Glycoprotein Using Human Monoclonal Antibodies from Phage
Display Libraries
Francesca
Bugli,1
Nicasio
Mancini,1
Chang-Yuil
Kang,2
Cristiana
Di
Campli,3
Antonella
Grieco,4
Aldo
Manzin,4
Armando
Gabrielli,5
Antonio
Gasbarrini,6
Giovanni
Fadda,1
Pietro E.
Varaldo,4
Massimo
Clementi,7 and
Roberto
Burioni4,*
Istituti di
Microbiologia,1 Clinica
Medica3 and Patologia
Medica,6 Facoltà di Medicina e Chirurgia,
Università Cattolica del S. Cuore, 00168 Rome, Istituti
di Microbiologia4 and Medicina
Interna,5 Facoltà di Medicina e Chirurgia,
Università di Ancona, 60020 Ancona, and Department of
Biomedical Sciences, Università di Trieste, Via Giorgieri 22,
34100 Trieste,7 Italy, and Laboratory of
Immunology, College of Pharmacy, Seoul National University, Seoul
151-742, Korea2
Received 16 April 2001/Accepted 12 July 2001
Clinical and experimental evidence indicates that the hepatitis C
virus (HCV) E2 glycoprotein (HCV/E2) is the most promising candidate
for the development of an effective anti-HCV vaccine. Identification of
the human epitopes that are conserved among isolates and are able to
elicit protective antibodies would constitute a significant step
forward. This work describes the mapping of the B-cell epitopes present
on the surface of HCV/E2, as recognized by the immune system during
infection, by the analysis of the reciprocal interactions of a panel of
human recombinant Fabs derived from an HCV-infected patient. Three
unrelated epitopes recognized by antibodies with no
neutralization-of-binding (NOB) activity were identified; a fourth,
major epitope was defined as a clustering of minor epitopes recognized
by Fabs endowed with strong NOB activity.
*
Corresponding author. Mailing address: Servizio di
Virologia, Istituto di Microbiologia, Facoltà di Medicina e
Chirurgia, Università di Ancona, Via Conca, 60020 ANCONA, Italy.
Phone: 39 071 5964849. Fax: 39 071 5964852. E-mail:
r.burioni{at}libero.it.
Journal of Virology, October 2001, p. 9986-9990, Vol. 75, No. 20
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.20.9986-9990.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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