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Journal of Virology, October 2001, p. 9819-9827, Vol. 75, No. 20
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.20.9819-9827.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

African Swine Fever Virus Protein p54 Interacts with the Microtubular Motor Complex through Direct Binding to Light-Chain Dynein

Covadonga Alonso,1,* James Miskin,2 Bruno Hernáez,1 Patricia Fernandez-Zapatero,1 Lourdes Soto,1 Carmen Cantó,1 Ignacio Rodríguez-Crespo,3 Linda Dixon,2 and José M. Escribano1

Departamento de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA),1 and Departamento de Bioquímica y Biología Molecular, Facultad de Químicas, Universidad Complutense, Madrid,3 Spain, and Institute for Animal Health, Pirbright GU24 ONF, United Kingdom2

Received 2 April 2001/Accepted 5 July 2001

Dynein is a minus-end-directed microtubule-associated motor protein involved in cargo transport in the cytoplasm. African swine fever virus (ASFV), a large DNA virus, hijacks the microtubule motor complex cellular transport machinery during virus infection of the cell through direct binding of virus protein p54 to the light chain of cytoplasmic dynein (LC8). Interaction of p54 and LC8 occurs both in vitro and in cells, and the two proteins colocalize at the microtubular organizing center during viral infection. p50/dynamitin, a dominant-negative inhibitor of dynein-dynactin function, impeded ASFV infection, suggesting an essential role for dynein during virus infection. A 13-amino-acid domain of p54 was sufficient for binding to LC8, an SQT motif within this domain being critical for this binding. Direct binding of a viral structural protein to LC8, a small molecule of the dynein motor complex, could constitute a molecular mechanism for microtubule-mediated virus transport.

* Corresponding author. Mailing address: Departamento de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Carretera de la Coruña Km 7, 28040 Madrid, Spain. Phone: 34-91-3476896. Fax: 34-91-3573107. E-mail: calonso{at}inia.es.

Journal of Virology, October 2001, p. 9819-9827, Vol. 75, No. 20
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.20.9819-9827.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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