JVI Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pult, I.
Right arrow Articles by Summers, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pult, I.
Right arrow Articles by Summers, J.

 Previous Article  |  Next Article 

Journal of Virology, October 2001, p. 9623-9632, Vol. 75, No. 20
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.20.9623-9632.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Frequency of Spontaneous Mutations in an Avian Hepadnavirus Infection

Irmgard Pult, Nathan Abbott, Yong-Yuan Zhang, and Jesse Summers*

Department of Molecular Genetics and Microbiology, The University of New Mexico School of Medicine, Albuquerque, New Mexico

Received 1 June 2001/Accepted 20 July 2001

In this study, we measured the frequency of revertants of a cytopathic strain of the duck hepatitis B virus that bears a single nucleotide substitution in the pre-S envelope protein open reading frame, resulting in the amino acid substitution G133E. Cytopathic virus mixed with known amounts of a genetically marked wild-type virus was injected into ducklings. Virus outgrowth was accompanied by a coselection of wild-type and spontaneous revertants during recovery of the ducklings from the acute liver injury caused by death of the G133E-infected cells. The frequency of individual revertants in the selected noncytopathic virus population was estimated by determining the ratio of each revertant to the wild-type virus. Spontaneous revertants were found to be present at frequencies of 1 × 10-5 to 6 × 10-5 per G133E genome inoculated. A mathematical model was used to estimate that the mutation rate was 0.8 × 10-5 to 4.5 × 10-5 per nucleotide per generation.

* Corresponding author. Mailing address: Department of Molecular Genetics and Microbiology, The University of New Mexico, 900 Camino de Salud, Albuquerque, NM 87131. Phone and fax: (505) 272-8896. E-mail: jsummer{at}unm.edu.

Journal of Virology, October 2001, p. 9623-9632, Vol. 75, No. 20
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.20.9623-9632.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Mol. Cell. Biol. Microbiol. Mol. Biol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2001 by the American Society for Microbiology. All rights reserved.