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Journal of Virology, October 2001, p. 10000-10004, Vol. 75, No. 20
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.20.10000-10004.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Arginine-Glycine-Aspartic Acid Motif Is Critical
for Human Parechovirus 1 Entry
Yingmanee
Boonyakiat,
Pamela J.
Hughes,
Farideh
Ghazi,
and
Glyn
Stanway*
Department of Biological Sciences, John Tabor
Laboratories, University of Essex, Colchester CO4 3SQ, United
Kingdom
Received 27 March 2001/Accepted 11 July 2001
The human parechovirus 1 RGD motif in VP1 was studied by
mutagenesis. An RGD-to-RGE change gave only revertant viruses with a
restored RGD, while deletion of GD was lethal and nonrevertable. Mutations at the +1 and +2 positions had some effect on growth properties and a +1 M-to-P change was lethal. These studies indicate that the RGD motif plays a critical role in infectivity, presumably by
interacting with integrins, and that downstream amino acids can have an
influence on function.
*
Corresponding author. Mailing address: Department of
Biological Sciences, Central Campus, University of Essex, Colchester CO4 3SQ, United Kingdom. Phone: 44 1206 873308. Fax: 44 1206 872592. E-mail: stanwg{at}essex.ac.uk.

Present address: Department of Biology, Faculty of Science, Chiang
Mai University, Chiang Mai 50200,
Thailand.

Present address: Department of Immunology and Microbiology, Iran
University Medical School, Tehran,
Iran.
Journal of Virology, October 2001, p. 10000-10004, Vol. 75, No. 20
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.20.10000-10004.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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