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Journal of Virology, October 2001, p. 10000-10004, Vol. 75, No. 20
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.20.10000-10004.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Arginine-Glycine-Aspartic Acid Motif Is Critical for Human Parechovirus 1 Entry

Yingmanee Boonyakiat,dagger Pamela J. Hughes, Farideh Ghazi,Dagger and Glyn Stanway*

Department of Biological Sciences, John Tabor Laboratories, University of Essex, Colchester CO4 3SQ, United Kingdom

Received 27 March 2001/Accepted 11 July 2001

The human parechovirus 1 RGD motif in VP1 was studied by mutagenesis. An RGD-to-RGE change gave only revertant viruses with a restored RGD, while deletion of GD was lethal and nonrevertable. Mutations at the +1 and +2 positions had some effect on growth properties and a +1 M-to-P change was lethal. These studies indicate that the RGD motif plays a critical role in infectivity, presumably by interacting with integrins, and that downstream amino acids can have an influence on function.

* Corresponding author. Mailing address: Department of Biological Sciences, Central Campus, University of Essex, Colchester CO4 3SQ, United Kingdom. Phone: 44 1206 873308. Fax: 44 1206 872592. E-mail: stanwg{at}essex.ac.uk.

dagger Present address: Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.

Dagger Present address: Department of Immunology and Microbiology, Iran University Medical School, Tehran, Iran.

Journal of Virology, October 2001, p. 10000-10004, Vol. 75, No. 20
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.20.10000-10004.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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