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Journal of Virology, January 2001, p. 910-920, Vol. 75, No. 2
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.2.910-920.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Comparison of Predicted Amino Acid Sequences of Measles Virus Strains in the Edmonston Vaccine Lineage

Christopher L. Parks, Robert A. Lerch, Pramila Walpita, Hai-Ping Wang, Mohinder S. Sidhu, and Stephen A. Udem*

Department of Viral Vaccine Research, Wyeth-Lederle Vaccines, Pearl River, New York 10965

Received 10 April 2000/Accepted 16 October 2000

Protein-encoding nucleotide sequences of the N, P, M, F, H, and L genes were determined for a low-passage isolate of the Edmonston wild-type (wt) measles virus and five Edmonston-derived vaccine virus strains, including AIK-C, Moraten, Schwarz, Rubeovax, and Zagreb. Comparative analysis demonstrated a high degree of nucleotide sequence homology; vaccine viruses differed at most by 0.3% from the Edmonston wt strain. Deduced amino acid sequences predicted substitutions in all viral polypetides. Eight amino acid coding changes were common to all vaccine viruses; an additional two were conserved in all vaccine strains except Zagreb. Comparisons made between vaccine strains indicated that commercial vaccine lots of Moraten and Schwarz had identical coding regions and were closely related to Rubeovax, while AIK-C and Zagreb diverged from the Edmonston wt along slightly different paths. These comparisons also revealed amino acid coding substitutions in Moraten and Schwarz that were absent from the closely related reactogenic Rubeovax strain. All of the vaccine viruses contained amino acid coding changes in the core components of the virus-encoded transcription and replication apparatus. This observation, combined with identification of noncoding region nucleotide changes in potential cis-acting sequences of the vaccine strains (C. L. Parks, R. A. Lerch, P. Walpita, H.-P. Wang, M. S. Sidhu, and S. A. Udem, J. Virol. 75:921-933, 2001), suggest that modulation of transcription and replication plays an important role in attenuation.

* Corresponding author. Mailing address: Department of Viral Vaccine Research, Wyeth-Lederle Vaccines, 401 North Middleton Rd., Pearl River, NY 10965. Phone: (845) 732-5450. Fax: (845) 732-5727. E-mail: udems{at}war.wyeth.com.

Journal of Virology, January 2001, p. 910-920, Vol. 75, No. 2
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.2.910-920.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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