This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Parks, C. L.
Right arrow Articles by Udem, S. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Parks, C. L.
Right arrow Articles by Udem, S. A.

 Previous Article  |  Next Article 

Journal of Virology, January 2001, p. 910-920, Vol. 75, No. 2
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.2.910-920.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Comparison of Predicted Amino Acid Sequences of Measles Virus Strains in the Edmonston Vaccine Lineage

Christopher L. Parks, Robert A. Lerch, Pramila Walpita, Hai-Ping Wang, Mohinder S. Sidhu, and Stephen A. Udem*

Department of Viral Vaccine Research, Wyeth-Lederle Vaccines, Pearl River, New York 10965

Received 10 April 2000/Accepted 16 October 2000

Protein-encoding nucleotide sequences of the N, P, M, F, H, and L genes were determined for a low-passage isolate of the Edmonston wild-type (wt) measles virus and five Edmonston-derived vaccine virus strains, including AIK-C, Moraten, Schwarz, Rubeovax, and Zagreb. Comparative analysis demonstrated a high degree of nucleotide sequence homology; vaccine viruses differed at most by 0.3% from the Edmonston wt strain. Deduced amino acid sequences predicted substitutions in all viral polypetides. Eight amino acid coding changes were common to all vaccine viruses; an additional two were conserved in all vaccine strains except Zagreb. Comparisons made between vaccine strains indicated that commercial vaccine lots of Moraten and Schwarz had identical coding regions and were closely related to Rubeovax, while AIK-C and Zagreb diverged from the Edmonston wt along slightly different paths. These comparisons also revealed amino acid coding substitutions in Moraten and Schwarz that were absent from the closely related reactogenic Rubeovax strain. All of the vaccine viruses contained amino acid coding changes in the core components of the virus-encoded transcription and replication apparatus. This observation, combined with identification of noncoding region nucleotide changes in potential cis-acting sequences of the vaccine strains (C. L. Parks, R. A. Lerch, P. Walpita, H.-P. Wang, M. S. Sidhu, and S. A. Udem, J. Virol. 75:921-933, 2001), suggest that modulation of transcription and replication plays an important role in attenuation.

* Corresponding author. Mailing address: Department of Viral Vaccine Research, Wyeth-Lederle Vaccines, 401 North Middleton Rd., Pearl River, NY 10965. Phone: (845) 732-5450. Fax: (845) 732-5727. E-mail: udems{at}war.wyeth.com.

Journal of Virology, January 2001, p. 910-920, Vol. 75, No. 2
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.2.910-920.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Devaux, P., Hodge, G., McChesney, M. B., Cattaneo, R. (2008). Attenuation of V- or C-Defective Measles Viruses: Infection Control by the Inflammatory and Interferon Responses of Rhesus Monkeys. J. Virol. 82: 5359-5367 [Abstract] [Full Text]  
  • Takeda, M., Tahara, M., Hashiguchi, T., Sato, T. A., Jinnouchi, F., Ueki, S., Ohno, S., Yanagi, Y. (2007). A Human Lung Carcinoma Cell Line Supports Efficient Measles Virus Growth and Syncytium Formation via a SLAM- and CD46-Independent Mechanism. J. Virol. 81: 12091-12096 [Abstract] [Full Text]  
  • del Valle, J. R., Devaux, P., Hodge, G., Wegner, N. J., McChesney, M. B., Cattaneo, R. (2007). A Vectored Measles Virus Induces Hepatitis B Surface Antigen Antibodies While Protecting Macaques against Measles Virus Challenge. J. Virol. 81: 10597-10605 [Abstract] [Full Text]  
  • Tahara, M., Takeda, M., Yanagi, Y. (2007). Altered Interaction of the Matrix Protein with the Cytoplasmic Tail of Hemagglutinin Modulates Measles Virus Growth by Affecting Virus Assembly and Cell-Cell Fusion. J. Virol. 81: 6827-6836 [Abstract] [Full Text]  
  • Tahara, M., Takeda, M., Seki, F., Hashiguchi, T., Yanagi, Y. (2007). Multiple Amino Acid Substitutions in Hemagglutinin Are Necessary for Wild-Type Measles Virus To Acquire the Ability To Use Receptor CD46 Efficiently. J. Virol. 81: 2564-2572 [Abstract] [Full Text]  
  • Yanagi, Y., Takeda, M., Ohno, S. (2006). Measles virus: cellular receptors, tropism and pathogenesis.. J. Gen. Virol. 87: 2767-2779 [Abstract] [Full Text]  
  • Seki, F., Takeda, M., Minagawa, H., Yanagi, Y. (2006). Recombinant wild-type measles virus containing a single N481Y substitution in its haemagglutinin cannot use receptor CD46 as efficiently as that having the haemagglutinin of the Edmonston laboratory strain. J. Gen. Virol. 87: 1643-1648 [Abstract] [Full Text]  
  • Carsillo, T., Zhang, X., Vasconcelos, D., Niewiesk, S., Oglesbee, M. (2006). A Single Codon in the Nucleocapsid Protein C Terminus Contributes to In Vitro and In Vivo Fitness of Edmonston Measles Virus. J. Virol. 80: 2904-2912 [Abstract] [Full Text]  
  • Waku-Kouomou, D., Alla, A., Blanquier, B., Jeantet, D., Caidi, H., Rguig, A., Freymuth, F., Wild, F. T. (2006). Genotyping Measles Virus by Real-Time Amplification Refractory Mutation System PCR Represents a Rapid Approach for Measles Outbreak Investigations. J. Clin. Microbiol. 44: 487-494 [Abstract] [Full Text]  
  • Tahara, M., Takeda, M., Yanagi, Y. (2005). Contributions of Matrix and Large Protein Genes of the Measles Virus Edmonston Strain to Growth in Cultured Cells as Revealed by Recombinant Viruses. J. Virol. 79: 15218-15225 [Abstract] [Full Text]  
  • Oldstone, M. B.A., Dales, S., Tishon, A., Lewicki, H., Martin, L. (2005). A role for dual viral hits in causation of subacute sclerosing panencephalitis. JEM 202: 1185-1190 [Abstract] [Full Text]  
  • Garcia, M., Yu, X.-F., Griffin, D. E., Moss, W. J. (2005). In Vitro Suppression of Human Immunodeficiency Virus Type 1 Replication by Measles Virus. J. Virol. 79: 9197-9205 [Abstract] [Full Text]  
  • Santibanez, S., Niewiesk, S., Heider, A., Schneider-Schaulies, J., Berbers, G. A. M., Zimmermann, A., Halenius, A., Wolbert, A., Deitemeier, I., Tischer, A., Hengel, H. (2005). Probing neutralizing-antibody responses against emerging measles viruses (MVs): immune selection of MV by H protein-specific antibodies?. J. Gen. Virol. 86: 365-374 [Abstract] [Full Text]  
  • Ohno, S., Ono, N., Takeda, M., Takeuchi, K., Yanagi, Y. (2004). Dissection of measles virus V protein in relation to its ability to block alpha/beta interferon signal transduction. J. Gen. Virol. 85: 2991-2999 [Abstract] [Full Text]  
  • Kingston, R. L., Baase, W. A., Gay, L. S. (2004). Characterization of Nucleocapsid Binding by the Measles Virus and Mumps Virus Phosphoproteins. J. Virol. 78: 8630-8640 [Abstract] [Full Text]  
  • Lorin, C., Mollet, L., Delebecque, F., Combredet, C., Hurtrel, B., Charneau, P., Brahic, M., Tangy, F. (2004). A Single Injection of Recombinant Measles Virus Vaccines Expressing Human Immunodeficiency Virus (HIV) Type 1 Clade B Envelope Glycoproteins Induces Neutralizing Antibodies and Cellular Immune Responses to HIV. J. Virol. 78: 146-157 [Abstract] [Full Text]  
  • Combredet, C., Labrousse, V., Mollet, L., Lorin, C., Delebecque, F., Hurtrel, B., McClure, H., Feinberg, M. B., Brahic, M., Tangy, F. (2003). A Molecularly Cloned Schwarz Strain of Measles Virus Vaccine Induces Strong Immune Responses in Macaques and Transgenic Mice. J. Virol. 77: 11546-11554 [Abstract] [Full Text]  
  • Kaye, S. (2003). Simple PCR-based Method for Synthesis of Molecular Calibrators and Controls. Clin. Chem. 49: 325-327 [Full Text]  
  • Pfeuffer, J., Puschel, K., Meulen, V. t., Schneider-Schaulies, J., Niewiesk, S. (2002). Extent of Measles Virus Spread and Immune Suppression Differentiates between Wild-Type and Vaccine Strains in the Cotton Rat Model (Sigmodon hispidus). J. Virol. 77: 150-158 [Abstract] [Full Text]  
  • Badgett, M. R., Auer, A., Carmichael, L. E., Parrish, C. R., Bull, J. J. (2002). Evolutionary Dynamics of Viral Attenuation. J. Virol. 76: 10524-10529 [Abstract] [Full Text]  
  • Bankamp, B., Kearney, S. P., Liu, X., Bellini, W. J., Rota, P. A. (2002). Activity of Polymerase Proteins of Vaccine and Wild-Type Measles Virus Strains in a Minigenome Replication Assay. J. Virol. 76: 7073-7081 [Abstract] [Full Text]  
  • Christiansen, D., De Sousa, E. R., Loveland, B., Kyriakou, P., Lanteri, M., Wild, F. T., Gerlier, D. (2002). A CD46CD[55-46] chimeric receptor, eight short consensus repeats long, acts as an inhibitor of both CD46 (MCP)- and CD150 (SLAM)-mediated cell-cell fusion induced by CD46-using measles virus. J. Gen. Virol. 83: 1147-1155 [Abstract] [Full Text]  
  • Plemper, R. K., Hammond, A. L., Gerlier, D., Fielding, A. K., Cattaneo, R. (2002). Strength of Envelope Protein Interaction Modulates Cytopathicity of Measles Virus. J. Virol. 76: 5051-5061 [Abstract] [Full Text]  
  • Waku Kouomou, D., Wild, T. F. (2002). Adaptation of Wild-Type Measles Virus to Tissue Culture. J. Virol. 76: 1505-1509 [Abstract] [Full Text]  
  • Parks, C. L., Lerch, R. A., Walpita, P., Wang, H.-P., Sidhu, M. S., Udem, S. A. (2001). Analysis of the Noncoding Regions of Measles Virus Strains in the Edmonston Vaccine Lineage. J. Virol. 75: 921-933 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»