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Journal of Virology, January 2001, p. 891-902, Vol. 75, No. 2
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.2.891-902.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Kaposi's Sarcoma-Associated Herpesvirus Latent and Lytic Gene Expression as Revealed by DNA Arrays

Richard G. Jenner,1 M. Mar Albà,1 Chris Boshoff,1,2 and Paul Kellam1,*

Wohl Virion Centre, Department of Immunology and Molecular Pathology, Windeyer Institute, University College London, London W1T 4JF,1 and Cancer Research Campaign Viral Oncology Group, Department of Oncology, Wolfson Institute for Biomedical Research, University College London, London WC1E 6AE,2 United Kingdom

Received 16 August 2000/Accepted 10 October 2000

Kaposi's sarcoma-associated herpesvirus (KSHV; human herpesvirus 8) is associated with three human tumors, Kaposi's sarcoma, primary effusion lymphoma (PEL), and multicentric Castleman's disease. KSHV encodes a number of homologs of cellular proteins involved in the cell cycle, signal transduction, and modulation of the host immune response. Of the virus complement of over 85 open reading frames (ORFs), the expression of only a minority has been characterized individually. We have constructed a nylon membrane-based DNA array which allows the expression of almost every ORF of KSHV to be measured simultaneously. A PEL-derived cell line, BC-3, was used to study the expression of KSHV during latency and after the induction of lytic replication. Cluster analysis, which arranges genes according to their expression profile, revealed a correlation between expression and assigned gene function that is consistent with the known stages of the herpesvirus life cycle. Furthermore, latent and lytic genes thought to be functionally related cluster into groups. The correlation between gene expression and function also infers possible roles for KSHV genes yet to be characterized.

* Corresponding author. Mailing address: Wohl Virion Centre, Windeyer Institute, University College London, 46 Cleveland St., London W1T 4JF , United Kingdom. Phone: 44 20 7679 9559. Fax: 44 20 7679 9555. E-mail: p.kellam{at}ucl.ac.uk.

Journal of Virology, January 2001, p. 891-902, Vol. 75, No. 2
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.2.891-902.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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