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Journal of Virology, January 2001, p. 878-890, Vol. 75, No. 2
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.2.878-890.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Inducible Expression of Inflammatory Chemokines in Respiratory Syncytial Virus-Infected Mice: Role of MIP-1alpha in Lung Pathology

Helene A. Haeberle,1,2 William A. Kuziel,3 Hans-Juergen Dieterich,2 Antonella Casola,1 Zoran Gatalica,4 and Roberto P. Garofalo1,5,*

Departments of Pediatrics,1 Pathology,4 and Microbiology and Immunology,5 The University of Texas Medical Branch, Galveston, and Department of Genetics and Microbiology, University of Texas, Austin,3 Texas, and Department of Anesthesiology, Universitaetsklinikum, Tuebingen, Germany2

Received 15 May 2000/Accepted 13 October 2000

Lower respiratory tract disease caused by respiratory syncytial virus (RSV) is characterized by profound airway mucosa inflammation, both in infants with naturally acquired infection and in experimentally inoculated animal models. Chemokines are central regulatory molecules in inflammatory, immune, and infectious processes of the lung. In this study, we demonstrate that intranasal infection of BALB/c mice with RSV A results in inducible expression of lung chemokines belonging to the CXC (MIP-2 and IP-10), CC (RANTES, eotaxin, MIP-1beta , MIP-1alpha , MCP-1, TCA-3) and C (lymphotactin) families. Chemokine mRNA expression occurred as early as 24 h following inoculation and persisted for at least 5 days in mice inoculated with the highest dose of virus (107 PFU). In general, levels of chemokine mRNA and protein were dependent on the dose of RSV inoculum and paralleled the intensity of lung cellular inflammation. Immunohisthochemical studies indicated that RSV-induced expression of MIP-1alpha , one of the most abundantly expressed chemokines, was primarily localized in epithelial cells of the alveoli and bronchioles, as well as in adjoining capillary endothelium. Genetically altered mice with a selective deletion of the MIP-1alpha gene (-/- mice) demonstrated a significant reduction in lung inflammation following RSV infection, compared to control littermates (+/+ mice). Despite the paucity of infiltrating cells, the peak RSV titer in the lung of -/- mice was not significantly different from that observed in +/+ mice. These results provide the first direct evidence that RSV infection may induce lung inflammation via the early production of inflammatory chemokines.

* Corresponding author. Mailing address: Division of Immunology/Allergy/Rheumatology, 301 University Blvd., Galveston, TX 77555-0369. Phone: (409) 772-2658. Fax: (409) 772-1761. E-mail: rpgarofa{at}utmb.edu.

Journal of Virology, January 2001, p. 878-890, Vol. 75, No. 2
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.2.878-890.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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