This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mortreux, F.
Right arrow Articles by Wattel, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mortreux, F.
Right arrow Articles by Wattel, E.

 Previous Article  |  Next Article 

Journal of Virology, January 2001, p. 1083-1089, Vol. 75, No. 2
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.2.1083-1089.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Two-Step Nature of Human T-Cell Leukemia Virus Type 1 Replication in Experimentally Infected Squirrel Monkeys (Saimiri sciureus)

Franck Mortreux,1,2 Mirdad Kazanji,3 Anne-Sophie Gabet,2 Benoit de Thoisy,4 and Eric Wattel2,*

Unité 524 INSERM, Institut de Recherche sur le Cancer de Lille, Lille,1 Unité d'Oncogenèse Virale, UMR5537 CNRS-Université Claude Bernard, Centre Léon Bérard, Lyon,2 France, and Laboratoire de Rétrovirologie3 and Centre de Primatologie,4 Institut Pasteur de la Guyane, Cayenne, Guyane Française

Received 11 August 2000/Accepted 25 October 2000

After experimental infection of squirrel monkeys (Saimiri sciureus) with human T-cell leukemia virus type 1 (HTLV-1)-infected cells, the virus is transcribed only transiently in circulating blood, spleen, and lymph nodes. Stable disappearance of viral expression occurs at 2 to 3 weeks after inoculation. This coincides with the development of the anti-HTLV-1 immune response and persistent detection of the provirus in peripheral blood mononuclear cells (PBMCs). In this study, the HTLV-1 replication pattern was analyzed over time in PBMCs and various organs from two HTLV-1-infected squirrel monkeys. Real-time quantitative PCR confirmed that PBMCs and lymphoid organs constitute the major reservoirs for HTLV-1. The PCR amplification of HTLV-1 flanking sequences from PBMCs evidenced a pattern of clonal expansion of infected cells identical to that observed in humans. Dissemination of the virus in body compartments appeared to result from cellular transport of the integrated provirus. The circulating proviral burden increased as a function of time in one animal studied over a period of 4 years. The high proviral loads observed in the last samples resulted from the accumulation of infected cells via the extensive proliferation of a restricted number of persistent clones on a background of polyclonally expanded HTLV-1-positive cells. Therefore, HTLV-1 primary infection in squirrel monkeys is a two-step process involving a transient phase of reverse transcription followed by persistent multiplication of infected cells. This suggests that the choice of the target for blocking HTLV-1 replication might depend on the stage of infection.

* Corresponding author. Mailing address: Unité d'Oncogenèse Virale, UMR5537-CNRS, Université Claude Bernard, Centre Léon-Bérard, 28, rue Laënnec, 69373 Lyon cedex 08, France. Phone: 334 78 78 26 69. Fax: 334 78 78 27 17. E-mail: wattel{at}lyon.fnclcc.fr.

Journal of Virology, January 2001, p. 1083-1089, Vol. 75, No. 2
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.2.1083-1089.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kchour, G., Tarhini, M., Kooshyar, M.-M., El Hajj, H., Wattel, E., Mahmoudi, M., Hatoum, H., Rahimi, H., Maleki, M., Rafatpanah, H., Rezaee, S. A. R., Yazdi, M. T., Shirdel, A., de The, H., Hermine, O., Farid, R., Bazarbachi, A. (2009). Phase 2 study of the efficacy and safety of the combination of arsenic trioxide, interferon alpha, and zidovudine in newly diagnosed chronic adult T-cell leukemia/lymphoma (ATL). Blood 113: 6528-6532 [Abstract] [Full Text]  
  • Besson, G., Kazanji, M. (2009). One-Step, Multiplex, Real-Time PCR Assay with Molecular Beacon Probes for Simultaneous Detection, Differentiation, and Quantification of Human T-Cell Leukemia Virus Types 1, 2, and 3. J. Clin. Microbiol. 47: 1129-1135 [Abstract] [Full Text]  
  • Rabaaoui, S., Zouhiri, F., Lancon, A., Leh, H., d'Angelo, J., Wattel, E. (2008). Inhibitors of Strand Transfer That Prevent Integration and Inhibit Human T-Cell Leukemia Virus Type 1 Early Replication. Antimicrob. Agents Chemother. 52: 3532-3541 [Abstract] [Full Text]  
  • Kazanji, M., Heraud, J.-M., Merien, F., Pique, C., de The, G., Gessain, A., Jacobson, S. (2006). Chimeric peptide vaccine composed of B- and T-cell epitopes of human T-cell leukemia virus type 1 induces humoral and cellular immune responses and reduces the proviral load in immunized squirrel monkeys (Saimiri sciureus).. J. Gen. Virol. 87: 1331-1337 [Abstract] [Full Text]  
  • Kim, S.-j., Nair, A. M., Fernandez, S., Mathes, L., Lairmore, M. D. (2006). Enhancement of LFA-1-Mediated T Cell Adhesion by Human T Lymphotropic Virus Type 1 p12I1. J. Immunol. 176: 5463-5470 [Abstract] [Full Text]  
  • Lemey, P., Van Dooren, S., Vandamme, A.-M. (2005). Evolutionary Dynamics of Human Retroviruses Investigated Through Full-Genome Scanning. Mol Biol Evol 22: 942-951 [Abstract] [Full Text]  
  • Bangham, C. R. M. (2003). The immune control and cell-to-cell spread of human T-lymphotropic virus type 1. J. Gen. Virol. 84: 3177-3189 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»