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Journal of Virology, January 2001, p. 1061-1064, Vol. 75, No. 2
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.2.1061-1064.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Recombinant Expression of Lymphocytic Choriomeningitis Virus Strain WE Glycoproteins: a Single Amino Acid Makes the Difference

Winfried R. Beyer, Hrvoje Miletic, Wolfram Ostertag, and Dorothee von Laer*

Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, D-20251 Hamburg, Germany

Received 8 August 2000/Accepted 25 October 2000

Cytoplasmic vector systems are generally used for expression of lymphocytic choriomeningitis virus (LCMV) proteins. However, we achieved high levels of cell surface glycoproteins using a standard nuclear expression plasmid. Expression was independent of other LCMV proteins but was blocked by a missense mutation within the original LCMV(WE) glycoprotein cDNA.

* Corresponding author. Present address: Georg-Speyer-Haus, Paul-Ehrlich-Strasse 42-44, D-60596 Frankfurt, Germany. Phone: 49-69-63395232. Fax: 49-69-63395297. E-mail: laer{at}em.uni-frankfurt.de.

Journal of Virology, January 2001, p. 1061-1064, Vol. 75, No. 2
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.2.1061-1064.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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