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Journal of Virology, October 2001, p. 9549-9552, Vol. 75, No. 19
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.19.9549-9552.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Characterization of Retrovirus-Host DNA Junctions in Cells Deficient in Nonhomologous-End Joining

Konstantin Taganov,1,2 René Daniel,1 Richard A. Katz,1 Olga Favorova,2 and Anna Marie Skalka1,*

Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111,1 and Department of Molecular Biology and Biotechnology, Russian State Medical University, Moscow, Russian Federation2

Received 20 February 2001/Accepted 23 June 2001

Formation of stably integrated proviruses is inefficient in cells that are defective in the cellular nonhomologous end-joining (NHEJ) DNA repair pathway (R. Daniel, R. A. Katz, and A. M. Skalka, Science 284:644-647, 1999; R. Daniel, R. A. Katz, and A. M. Skalka, Mol. Cell. Biol. 21:1164-1172, 2001). However, the requirement for NHEJ function is not absolute, as 10 to 20% of infected NHEJ-deficient cells can express retrovirus- transduced reporter genes in a stable fashion. To learn more about the compensatory mechanism by which viral DNA may be incorporated into the host cell genome, we analyzed the nucleotide sequences of provirus-host DNA junctions in singly infected NHEJ-deficient cell clones. The results showed that the proviral DNA ends in all NHEJ-deficient clones had the normal 5'TG ... CA3' sequence. In addition, 14 of the 19 proviruses analyzed were flanked by a 6-bp direct repeat of host sequences, as is characteristic for avian sarcoma virus integration. These results indicate that the DNA repair pathway which compensates for loss of NHEJ in these transductants does not introduce any gross abnormalities at the provirus-host DNA junctions.

* Corresponding author. Mailing address: Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Ave., Philadelphia, PA 19111. Phone: (215) 728-2490. Fax: (215) 728-2778. E-mail: AM_Skalka{at}fccc.edu.

Journal of Virology, October 2001, p. 9549-9552, Vol. 75, No. 19
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.19.9549-9552.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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