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Journal of Virology, October 2001, p. 9543-9548, Vol. 75, No. 19
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.19.9543-9548.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Analysis of Mason-Pfizer Monkey Virus Gag Particles by Scanning Transmission Electron Microscopy

Scott D. Parker,1 Joseph S. Wall,2 and Eric Hunter3,*

Department of Medicine, Division of Infectious Diseases,1 and Department of Microbiology,3 University of Alabama at Birmingham, Birmingham, Alabama 35294, and Department of Biology, Brookhaven National Laboratories, Upton, New York 119732

Received 6 April 2001/Accepted 29 June 2001

Mason-Pfizer monkey virus immature capsids selected from the cytoplasm of baculovirus-infected cells were imaged by scanning transmission electron microscopy. The masses of individual selected Gag particles were measured, and the average mass corresponded to 1,900 to 2,100 Gag polyproteins per particle. A large variation in Gag particle mass was observed within each population measured.

* Corresponding author. Mailing address: Department of Microbiology, University of Alabama at Birmingham, BBRB 256, 1530 3rd Ave. South, Birmingham, AL 35294. Phone: (205) 934-4321. Fax: (205) 934-1640. E-mail: Ehunter{at}UAB.edu.

Journal of Virology, October 2001, p. 9543-9548, Vol. 75, No. 19
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.19.9543-9548.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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