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Journal of Virology, October 2001, p. 9538-9542, Vol. 75, No. 19
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.19.9538-9542.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

A Single Amino Acid Mutation in the Carnation Ringspot Virus Capsid Protein Allows Virion Formation but Prevents Systemic Infection

Tim L. Sit, Patrick R. Haikal,dagger Anton S. Callaway, and Steven A. Lommel*

Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27695-7616

Received 9 April 2001/Accepted 5 July 2001

A Carnation ringspot virus (CRSV) variant (1.26) was identified that accumulates virions but is incapable of forming a systemic infection. The 1.26 capsid protein gene possesses a Serright-arrowPro mutation at amino acid 282. Conversion of 1.26 amino acid 282 to Ser restored systemic infection, while the reciprocal mutation in wild-type CRSV abolished systemic infection. Similar mutations introduced into the related Red clover necrotic mosaic virus capsid protein gene failed to induce the packaging but nonsystemic movement phenotype. These results provide additional support for the theory that virion formation is necessary but not sufficient for systemic movement with the dianthoviruses.

* Corresponding author. Mailing address: Department of Plant Pathology, North Carolina State University, Raleigh, NC 27695-7616. Phone: (919) 515-6990. Fax: (919) 515-7716. E-mail: steve_lommel{at}ncsu.edu.

dagger Present address: Tomen Agro, Inc., San Francisco, CA 94105.

Journal of Virology, October 2001, p. 9538-9542, Vol. 75, No. 19
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.19.9538-9542.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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