This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rinck, G.
Right arrow Articles by Tautz, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rinck, G.
Right arrow Articles by Tautz, N.

 Previous Article  |  Next Article 

Journal of Virology, October 2001, p. 9470-9482, Vol. 75, No. 19
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.19.9470-9482.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

A Cellular J-Domain Protein Modulates Polyprotein Processing and Cytopathogenicity of a Pestivirus

G. Rinck,1,dagger C. Birghan,1 T. Harada,1,Dagger G. Meyers,2 H.-J. Thiel,1 and N. Tautz1,*

Institut für Virologie (FB Veterinärmedizin), Justus-Liebig-Universität Giessen, D-35392 Giessen,1 and Federal Research Center for Virus Diseases of Animals, D-72076 Tübingen,2 Germany

Received 5 April 2001/Accepted 21 June 2001

Pestiviruses are positive-strand RNA viruses closely related to human hepatitis C virus. Gene expression of these viruses occurs via translation of a polyprotein, which is further processed by cellular and viral proteases. Here we report the formation of a stable complex between an as-yet-undescribed cellular J-domain protein, a member of the DnaJ-chaperone family, and pestiviral nonstructural protein NS2. Accordingly, we termed the cellular protein Jiv, for J-domain protein interacting with viral protein. Jiv has the potential to induce in trans one specific processing step in the viral polyprotein, namely, cleavage of NS2-3. Efficient generation of its cleavage product NS3 has previously been shown to be obligatory for the cytopathogenicity of the pestiviruses. Regulated expression of Jiv in cells infected with noncytopathogenic bovine viral diarrhea virus disclosed a direct correlation between the intracellular level of Jiv, the extent of NS2-3 cleavage, and pestiviral cytopathogenicity.

* Corresponding author. Mailing address: Institut für Virologie (FB Veterinärmedizin), Justus-Liebig-Universität Giessen, Frankfurter Strasse 107, D-35392 Giessen, Germany. Phone: 49 (641) 99-38375. Fax: 49 (641) 99-38359. E-mail: Norbert.Tautz{at}vetmed.uni-giessen.de.

dagger Present address: Virco UK, Ltd., Cambridge, United Kingdom.

Dagger Present address: Chiron Corp., I-53100 Siena, Italy.

Journal of Virology, October 2001, p. 9470-9482, Vol. 75, No. 19
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.19.9470-9482.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Gallei, A., Blome, S., Gilgenbach, S., Tautz, N., Moennig, V., Becher, P. (2008). Cytopathogenicity of Classical Swine Fever Virus Correlates with Attenuation in the Natural Host. J. Virol. 82: 9717-9729 [Abstract] [Full Text]  
  • Afonso, C. L., Tulman, E. R., Delhon, G., Lu, Z., Viljoen, G. J., Wallace, D. B., Kutish, G. F., Rock, D. L. (2006). Genome of crocodilepox virus.. J. Virol. 80: 4978-4991 [Abstract] [Full Text]  
  • Krey, T., Himmelreich, A., Heimann, M., Menge, C., Thiel, H.-J., Maurer, K., Rumenapf, T. (2006). Function of Bovine CD46 as a Cellular Receptor for Bovine Viral Diarrhea Virus Is Determined by Complement Control Protein 1.. J. Virol. 80: 3912-3922 [Abstract] [Full Text]  
  • Heimann, M., Sosa, G. R., Martoglio, B., Thiel, H.-J., Rumenapf, T. (2006). Core Protein of Pestiviruses Is Processed at the C Terminus by Signal Peptide Peptidase. J. Virol. 80: 1915-1921 [Abstract] [Full Text]  
  • Lackner, T., Thiel, H.-J., Tautz, N. (2006). Dissection of a viral autoprotease elucidates a function of a cellular chaperone in proteolysis. Proc. Natl. Acad. Sci. USA 103: 1510-1515 [Abstract] [Full Text]  
  • Wieland, S. F., Chisari, F. V. (2005). Stealth and Cunning: Hepatitis B and Hepatitis C Viruses. J. Virol. 79: 9369-9380 [Full Text]  
  • Lackner, T., Muller, A., Konig, M., Thiel, H.-J., Tautz, N. (2005). Persistence of Bovine Viral Diarrhea Virus Is Determined by a Cellular Cofactor of a Viral Autoprotease. J. Virol. 79: 9746-9755 [Abstract] [Full Text]  
  • Schelle, B., Karl, N., Ludewig, B., Siddell, S. G., Thiel, V. (2005). Selective Replication of Coronavirus Genomes That Express Nucleocapsid Protein. J. Virol. 79: 6620-6630 [Abstract] [Full Text]  
  • Krey, T., Thiel, H.-J., Rumenapf, T. (2005). Acid-Resistant Bovine Pestivirus Requires Activation for pH-Triggered Fusion during Entry. J. Virol. 79: 4191-4200 [Abstract] [Full Text]  
  • Coley, S. E., Lavi, E., Sawicki, S. G., Fu, L., Schelle, B., Karl, N., Siddell, S. G., Thiel, V. (2005). Recombinant Mouse Hepatitis Virus Strain A59 from Cloned, Full-Length cDNA Replicates to High Titers In Vitro and Is Fully Pathogenic In Vivo. J. Virol. 79: 3097-3106 [Abstract] [Full Text]  
  • Lackner, T., Muller, A., Pankraz, A., Becher, P., Thiel, H.-J., Gorbalenya, A. E., Tautz, N. (2004). Temporal Modulation of an Autoprotease Is Crucial for Replication and Pathogenicity of an RNA Virus. J. Virol. 78: 10765-10775 [Abstract] [Full Text]  
  • Fricke, J., Voss, C., Thumm, M., Meyers, G. (2004). Processing of a Pestivirus Protein by a Cellular Protease Specific for Light Chain 3 of Microtubule-Associated Proteins. J. Virol. 78: 5900-5912 [Abstract] [Full Text]  
  • Agapov, E. V., Murray, C. L., Frolov, I., Qu, L., Myers, T. M., Rice, C. M. (2004). Uncleaved NS2-3 Is Required for Production of Infectious Bovine Viral Diarrhea Virus. J. Virol. 78: 2414-2425 [Abstract] [Full Text]  
  • Muller, A., Rinck, G., Thiel, H.-J., Tautz, N. (2003). Cell-Derived Sequences in the N-Terminal Region of the Polyprotein of a Cytopathogenic Pestivirus. J. Virol. 77: 10663-10669 [Abstract] [Full Text]  
  • Nagai, M., Sakoda, Y., Mori, M., Hayashi, M., Kida, H., Akashi, H. (2003). Insertion of cellular sequence and RNA recombination in the structural protein coding region of cytopathogenic bovine viral diarrhoea virus. J. Gen. Virol. 84: 447-452 [Abstract] [Full Text]  
  • Jordan, R., Wang, L., Graczyk, T. M., Block, T. M., Romano, P. R. (2002). Replication of a Cytopathic Strain of Bovine Viral Diarrhea Virus Activates PERK and Induces Endoplasmic Reticulum Stress-Mediated Apoptosis of MDBK Cells. J. Virol. 76: 9588-9599 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»