This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cornu, T. I.
Right arrow Articles by de la Torre, J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cornu, T. I.
Right arrow Articles by de la Torre, J. C.

 Previous Article  |  Next Article 

Journal of Virology, October 2001, p. 9415-9426, Vol. 75, No. 19
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.19.9415-9426.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

RING Finger Z Protein of Lymphocytic Choriomeningitis Virus (LCMV) Inhibits Transcription and RNA Replication of an LCMV S-Segment Minigenome†

Tatjana I. Cornu and Juan Carlos de la Torre*

Division of Virology, Department of Neuropharmacology, The Scripps Research Institute, La Jolla, California 92037

Received 26 January 2001/Accepted 22 June 2001

Arenaviruses have a bisegmented negative-strand RNA genome whose proteomic capability is limited to only four polypeptides, namely, nucleoprotein (NP), surface glycoprotein (GP) that is proteolytically processed into GP1+GP2, polymerase (L), and a small (11-kDa) RING finger protein (Z). The role of Z during the Lymphocytic choriomeningitis virus (LCMV) life cycle is poorly understood. We investigated the function of Z in virus transcription and replication by using a reverse genetic system for the prototypic arenavirus LCMV. This system involves an LCMV minigenome and the minimal viral trans-acting factors (NP and L), expressed from separated cotransfected plasmids. Cotransfection of the Z cDNA strongly inhibited LCMV minigenome expression. The effect required synthesis of Z protein; its magnitude was dose dependent and occurred with levels of Z protein substantially lower than those observed in LCMV-infected cells. Coexpression of Z did not prevent the encapsidation of plasmid supplied minigenome, but it affected both transcription and RNA replication similarly. Mutations in Z that unfolded its RING finger domain eliminated its inhibitory activity, but RING proteins not related to Z did not affect LCMV minigenome expression. Consistent with the minigenome results, cells transiently expressing Z exhibited decreased susceptibility to infection with LCMV.

* Corresponding author. Mailing address: The Scripps Research Institute, Department of Neuropharmacology, IMM-6, 10550 N. Torrey Pines Rd., La Jolla, CA 92037. Phone: (858) 784-9462. Fax: (858) 784-9981. E-mail: juanct{at}scripps.edu.

dagger Publication 13869-NP from The Scripps Research Institute.

Journal of Virology, October 2001, p. 9415-9426, Vol. 75, No. 19
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.19.9415-9426.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Casabona, J. C., Levingston Macleod, J. M., Loureiro, M. E., Gomez, G. A., Lopez, N. (2009). The RING Domain and the L79 Residue of Z Protein Are Involved in both the Rescue of Nucleocapsids and the Incorporation of Glycoproteins into Infectious Chimeric Arenavirus-Like Particles. J. Virol. 83: 7029-7039 [Abstract] [Full Text]  
  • Baize, S., Marianneau, P., Loth, P., Reynard, S., Journeaux, A., Chevallier, M., Tordo, N., Deubel, V., Contamin, H. (2009). Early and Strong Immune Responses Are Associated with Control of Viral Replication and Recovery in Lassa Virus-Infected Cynomolgus Monkeys. J. Virol. 83: 5890-5903 [Abstract] [Full Text]  
  • Neumann, P., Lieber, D., Meyer, S., Dautel, P., Kerth, A., Kraus, I., Garten, W., Stubbs, M. T. (2009). Crystal structure of the Borna disease virus matrix protein (BDV-M) reveals ssRNA binding properties. Proc. Natl. Acad. Sci. USA 106: 3710-3715 [Abstract] [Full Text]  
  • Wilda, M., Lopez, N., Casabona, J. C., Franze-Fernandez, M. T. (2008). Mapping of the Tacaribe Arenavirus Z-Protein Binding Sites on the L Protein Identified both Amino Acids within the Putative Polymerase Domain and a Region at the N Terminus of L That Are Critically Involved in Binding. J. Virol. 82: 11454-11460 [Abstract] [Full Text]  
  • Ellenberg, P., Linero, F. N., Scolaro, L. A. (2007). Superinfection exclusion in BHK-21 cells persistently infected with Junin virus. J. Gen. Virol. 88: 2730-2739 [Abstract] [Full Text]  
  • Capul, A. A., Perez, M., Burke, E., Kunz, S., Buchmeier, M. J., de la Torre, J. C. (2007). Arenavirus Z-Glycoprotein Association Requires Z Myristoylation but Not Functional RING or Late Domains. J. Virol. 81: 9451-9460 [Abstract] [Full Text]  
  • Chase, G., Mayer, D., Hildebrand, A., Frank, R., Hayashi, Y., Tomonaga, K., Schwemmle, M. (2007). Borna Disease Virus Matrix Protein Is an Integral Component of the Viral Ribonucleoprotein Complex That Does Not Interfere with Polymerase Activity. J. Virol. 81: 743-749 [Abstract] [Full Text]  
  • Kunz, S., Rojek, J. M., Roberts, A. J., McGavern, D. B., Oldstone, M. B. A., de la Torre, J. C. (2006). Altered central nervous system gene expression caused by congenitally acquired persistent infection with lymphocytic choriomeningitis virus.. J. Virol. 80: 9082-9092 [Abstract] [Full Text]  
  • Garcia, C. C., Djavani, M., Topisirovic, I., Borden, K. L. B., Salvato, M. S., Damonte, E. B. (2006). Arenavirus Z protein as an antiviral target: virus inactivation and protein oligomerization by zinc finger-reactive compounds.. J. Gen. Virol. 87: 1217-1228 [Abstract] [Full Text]  
  • Sanchez, A. B., Perez, M., Cornu, T., de la Torre, J. C. (2005). RNA Interference-Mediated Virus Clearance from Cells both Acutely and Chronically Infected with the Prototypic Arenavirus Lymphocytic Choriomeningitis Virus. J. Virol. 79: 11071-11081 [Abstract] [Full Text]  
  • Sanchez, A. B., de la Torre, J. C. (2005). Genetic and Biochemical Evidence for an Oligomeric Structure of the Functional L Polymerase of the Prototypic Arenavirus Lymphocytic Choriomeningitis Virus. J. Virol. 79: 7262-7268 [Abstract] [Full Text]  
  • Pinschewer, D. D., Perez, M., de la Torre, J. C. (2005). Dual Role of the Lymphocytic Choriomeningitis Virus Intergenic Region in Transcription Termination and Virus Propagation. J. Virol. 79: 4519-4526 [Abstract] [Full Text]  
  • Neuman, B. W., Adair, B. D., Burns, J. W., Milligan, R. A., Buchmeier, M. J., Yeager, M. (2005). Complementarity in the Supramolecular Design of Arenaviruses and Retroviruses Revealed by Electron Cryomicroscopy and Image Analysis. J. Virol. 79: 3822-3830 [Abstract] [Full Text]  
  • Hass, M., Golnitz, U., Muller, S., Becker-Ziaja, B., Gunther, S. (2004). Replicon System for Lassa Virus. J. Virol. 78: 13793-13803 [Abstract] [Full Text]  
  • Perez, M., Greenwald, D. L., de La Torre, J. C. (2004). Myristoylation of the RING Finger Z Protein Is Essential for Arenavirus Budding. J. Virol. 78: 11443-11448 [Abstract] [Full Text]  
  • Cornu, T. I., Feldmann, H., de la Torre, J. C. (2004). Cells Expressing the RING Finger Z Protein Are Resistant to Arenavirus Infection. J. Virol. 78: 2979-2983 [Abstract] [Full Text]  
  • Baize, S., Kaplon, J., Faure, C., Pannetier, D., Georges-Courbot, M.-C., Deubel, V. (2004). Lassa Virus Infection of Human Dendritic Cells and Macrophages Is Productive but Fails to Activate Cells. J. Immunol. 172: 2861-2869 [Abstract] [Full Text]  
  • Perez, M., Craven, R. C., de la Torre, J. C. (2003). The small RING finger protein Z drives arenavirus budding: Implications for antiviral strategies. Proc. Natl. Acad. Sci. USA 100: 12978-12983 [Abstract] [Full Text]  
  • Jacamo, R., Lopez, N., Wilda, M., Franze-Fernandez, M. T. (2003). Tacaribe Virus Z Protein Interacts with the L Polymerase Protein To Inhibit Viral RNA Synthesis. J. Virol. 77: 10383-10393 [Abstract] [Full Text]  
  • Strecker, T., Eichler, R., Meulen, J. t., Weissenhorn, W., Dieter Klenk, H., Garten, W., Lenz, O. (2003). Lassa Virus Z Protein Is a Matrix Protein Sufficient for the Release of Virus-Like Particles. J. Virol. 77: 10700-10705 [Abstract] [Full Text]  
  • Pinschewer, D. D., Perez, M., Sanchez, A. B., de la Torre, J. C. (2003). Recombinant lymphocytic choriomeningitis virus expressing vesicular stomatitis virus glycoprotein. Proc. Natl. Acad. Sci. USA 100: 7895-7900 [Abstract] [Full Text]  
  • Pinschewer, D. D., Perez, M., de la Torre, J. C. (2003). Role of the Virus Nucleoprotein in the Regulation of Lymphocytic Choriomeningitis Virus Transcription and RNA Replication. J. Virol. 77: 3882-3887 [Abstract] [Full Text]  
  • Perez, M., de la Torre, J. C. (2002). Characterization of the Genomic Promoter of the Prototypic Arenavirus Lymphocytic Choriomeningitis Virus. J. Virol. 77: 1184-1194 [Abstract] [Full Text]  
  • Cornu, T. I., de la Torre, J. C. (2002). Characterization of the Arenavirus RING Finger Z Protein Regions Required for Z-Mediated Inhibition of Viral RNA Synthesis. J. Virol. 76: 6678-6688 [Abstract] [Full Text]  
  • Lee, K. J., Perez, M., Pinschewer, D. D., de la Torre, J. C. (2002). Identification of the Lymphocytic Choriomeningitis Virus (LCMV) Proteins Required To Rescue LCMV RNA Analogs into LCMV-Like Particles. J. Virol. 76: 6393-6397 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»