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Journal of Virology, September 2001, p. 8831-8836, Vol. 75, No. 18
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.18.8831-8836.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Tobamovirus Replicase Coding Region Is Involved in
Cell-to-Cell Movement
Kyotaro
Hirashima and
Yuichiro
Watanabe*
Department of Life Sciences, Graduate School
of Arts and Sciences, University of Tokyo, Meguro-ku, Tokyo
153-8902, Japan
Received 29 January 2001/Accepted 29 May 2001
Tobacco mosaic virus (TMV) encodes a 30-kDa movement
protein (MP) which enables viral movement from cell to cell. It is,
however, unclear whether the 126- and 183-kDa replicase proteins are
involved in the cell-to-cell movement of TMV. In the course of our
studies into TMV-R, a strain with a host range different from that of TMV-U1, we have obtained an interesting chimeric virus, UR-hel. The
amino acid sequence differences between UR-hel and TMV-U1 are located
only in the helicase-like domain of the replicase. Interestingly,
UR-hel has a defect in its cell-to-cell movement. The replication of
UR-hel showed a level of replication of the genome, synthesis, and
accumulation of MP similar to that observed in TMV-U1-inoculated
protoplasts. Such observations support the hypothesis that the
replicase coding region may in some fashion be involved in cell-to-cell
movement of TMV.
*
Corresponding author. Mailing address: Department of
Life Sciences, Graduate School of Arts and Sciences, University of
Tokyo, Komaba 3-8-1, Meguro-ku, Tokyo 153-8902, Japan. Phone:
81-3-5454-6776. Fax: 81-3-5454-6776. E-mail:
solan{at}bio.c.u-tokyo.ac.jp.
Journal of Virology, September 2001, p. 8831-8836, Vol. 75, No. 18
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.18.8831-8836.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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