This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lawrence, D. M.
Right arrow Articles by Jackson, A. O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lawrence, D. M.
Right arrow Articles by Jackson, A. O.

 Previous Article  |  Next Article 

Journal of Virology, September 2001, p. 8712-8723, Vol. 75, No. 18
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.18.8712-8723.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Interactions of the TGB1 Protein during Cell-to-Cell Movement of Barley Stripe Mosaic Virus

Diane M. Lawrence and A. O. Jackson*

Department of Plant and Microbial Biology, University of California, Berkeley, California 94720

Received 1 March 2001/Accepted 12 June 2001

We have recently used a green fluorescent protein (GFP) fusion to the gamma b protein of Barley stripe mosaic virus (BSMV) to monitor cell-to-cell and systemic virus movement. The gamma b protein is involved in expression of the triple gene block (TGB) proteins encoded by RNAbeta but is not essential for cell-to-cell movement. The GFP fusion appears not to compromise replication or movement substantially, and mutagenesis experiments demonstrated that the three most abundant TGB-encoded proteins, beta b (TGB1), beta c (TGB3), and beta d (TGB2), are each required for cell-to-cell movement (D. M. Lawrence and A. O. Jackson, Mol. Plant Pathol. 2:65-75, 2001). We have now extended these analyses by engineering a fusion of GFP to TGB1 to examine the expression and interactions of this protein during infection. BSMV derivatives containing the TGB1 fusion were able to move from cell to cell and establish local lesions in Chenopodium amaranticolor and systemic infections of Nicotiana benthamiana and barley. In these hosts, the GFP-TGB1 fusion protein exhibited a temporal pattern of expression along the advancing edge of the infection front. Microscopic examination of the subcellular localization of the GFP-TGB1 protein indicated an association with the endoplasmic reticulum and with plasmodesmata. The subcellular localization of the TGB1 protein was altered in infections in which site-specific mutations were introduced into the six conserved regions of the helicase domain and in mutants unable to express the TGB2 and/or TGB3 proteins. These results are compatible with a model suggesting that movement requires associations of the TGB1 protein with cytoplasmic membranes that are facilitated by the TGB2 and TGB3 proteins.

* Corresponding author. Mailing address: Department of Plant and Microbial Biology, 111 Koshland Hall, University of California, Berkeley, CA 94720. Phone: (510) 642-3906. Fax: (510) 642-9017. E-mail: andyoj{at}uclink4.berkeley.edu.

Journal of Virology, September 2001, p. 8712-8723, Vol. 75, No. 18
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.18.8712-8723.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lim, H.-S., Bragg, J. N., Ganesan, U., Lawrence, D. M., Yu, J., Isogai, M., Hammond, J., Jackson, A. O. (2008). Triple Gene Block Protein Interactions Involved in Movement of Barley Stripe Mosaic Virus. J. Virol. 82: 4991-5006 [Abstract] [Full Text]  
  • Schepetilnikov, M. V., Solovyev, A. G., Gorshkova, E. N., Schiemann, J., Prokhnevsky, A. I., Dolja, V. V., Morozov, S. Y. (2008). Intracellular Targeting of a Hordeiviral Membrane-Spanning Movement Protein: Sequence Requirements and Involvement of an Unconventional Mechanism. J. Virol. 82: 1284-1293 [Abstract] [Full Text]  
  • Garcia-Ruiz, H., Ahlquist, P. (2006). Inducible Yeast System for Viral RNA Recombination Reveals Requirement for an RNA Replication Signal on Both Parental RNAs.. J. Virol. 80: 8316-8328 [Abstract] [Full Text]  
  • Torrance, L., Cowan, G. H., Gillespie, T., Ziegler, A., Lacomme, C. (2006). Barley stripe mosaic virus-encoded proteins triple-gene block 2 and {gamma}b localize to chloroplasts in virus-infected monocot and dicot plants, revealing hitherto-unknown roles in virus replication.. J. Gen. Virol. 87: 2403-2411 [Abstract] [Full Text]  
  • Lee, J.-Y., Taoka, K.-i., Yoo, B.-C., Ben-Nissan, G., Kim, D.-J., Lucas, W. J. (2005). Plasmodesmal-Associated Protein Kinase in Tobacco and Arabidopsis Recognizes a Subset of Non-Cell-Autonomous Proteins. Plant Cell 17: 2817-2831 [Abstract] [Full Text]  
  • Schepetilnikov, M. V., Manske, U., Solovyev, A. G., Zamyatnin, A. A. Jr, Schiemann, J., Morozov, S. Yu. (2005). The hydrophobic segment of Potato virus X TGBp3 is a major determinant of the protein intracellular trafficking. J. Gen. Virol. 86: 2379-2391 [Abstract] [Full Text]  
  • Yelina, N. E., Erokhina, T. N., Lukhovitskaya, N. I., Minina, E. A., Schepetilnikov, M. V., Lesemann, D.-E., Schiemann, J., Solovyev, A. G., Morozov, S. Yu. (2005). Localization of Poa semilatent virus cysteine-rich protein in peroxisomes is dispensable for its ability to suppress RNA silencing. J. Gen. Virol. 86: 479-489 [Abstract] [Full Text]  
  • Haupt, S., Cowan, G. H., Ziegler, A., Roberts, A. G., Oparka, K. J., Torrance, L. (2005). Two Plant-Viral Movement Proteins Traffic in the Endocytic Recycling Pathway. Plant Cell 17: 164-181 [Abstract] [Full Text]  
  • Peremyslov, V. V., Pan, Y.-W., Dolja, V. V. (2004). Movement Protein of a Closterovirus Is a Type III Integral Transmembrane Protein Localized to the Endoplasmic Reticulum. J. Virol. 78: 3704-3709 [Abstract] [Full Text]  
  • Morozov, S. Yu., Solovyev, A. G. (2003). Triple gene block: modular design of a multifunctional machine for plant virus movement. J. Gen. Virol. 84: 1351-1366 [Abstract] [Full Text]  
  • Gorshkova, E. N., Erokhina, T. N., Stroganova, T. A., Yelina, N. E., Zamyatnin, A. A. Jr, Kalinina, N. O., Schiemann, J., Solovyev, A. G., Morozov, S. Yu. (2003). Immunodetection and fluorescent microscopy of transgenically expressed hordeivirus TGBp3 movement protein reveals its association with endoplasmic reticulum elements in close proximity to plasmodesmata. J. Gen. Virol. 84: 985-994 [Abstract] [Full Text]  
  • Zamyatnin, A. A. Jr,, Solovyev, A. G., Sablina, A. A., Agranovsky, A. A., Katul, L., Vetten, H. J., Schiemann, J., Hinkkanen, A. E., Lehto, K., Morozov, S. Yu. (2002). Dual-colour imaging of membrane protein targeting directed by poa semilatent virus movement protein TGBp3 in plant and mammalian cells. J. Gen. Virol. 83: 651-662 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»