This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Derse, D.
Right arrow Articles by Morse, B. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Derse, D.
Right arrow Articles by Morse, B. A.

 Previous Article  |  Next Article 

Journal of Virology, September 2001, p. 8461-8468, Vol. 75, No. 18
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.18.8461-8468.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Examining Human T-Lymphotropic Virus Type 1 Infection and Replication by Cell-Free Infection with Recombinant Virus Vectors

David Derse,1,* Shawn A. Hill,1 Patricia A. Lloyd,2 Hye-kyung Chung,1 and Barry A. Morse1

Basic Research Laboratory, National Cancer Institute, NCI-Frederick,1 and SAIC-Frederick,2 Frederick, Maryland 21702

Received 4 May 2001/Accepted 19 June 2001

A sensitive and quantitative cell-free infection assay, utilizing recombinant human T-cell leukemia virus type 1 (HTLV-1)-based vectors, was developed in order to analyze early events in the virus replication cycle. Previous difficulties with the low infectivity and restricted expression of the virus have prevented a clear understanding of these events. Virus stocks were generated by transfecting cells with three plasmids: (i) a packaging plasmid encoding HTLV-1 structural and regulatory proteins, (ii) an HTLV-1 transfer vector containing either firefly luciferase or enhanced yellow fluorescent protein genes, and (iii) an envelope expression plasmid. Single-round infections were initiated by exposing target cells to filtered supernatants and quantified by assaying for luciferase activity in cell extracts or by enumerating transduced cells by flow cytometry. Transduction was dependent on reverse transcription and integration of the recombinant virus genome, as shown by the effects of the reverse transcriptase inhibitor 3'-azido-3'-deoxythymidine (AZT) and by mutation of the integrase gene in the packaging vector, respectively. The 50% inhibitory concentration of AZT was determined to be 30 nM in this HTLV-1 replication system. The stability of HTLV-1 particles, pseudotyped with either vesicular stomatitis virus G protein or HTLV-1 envelope, was typical of retroviruses, exhibiting a half-life of approximately 3.5 h at 37°C. The specific infectivity of recombinant HTLV-1 virions was at least 3 orders of magnitude lower than that of analogous HIV-1 particles, though both were pseudotyped with the same envelope. Thus, the low infectivity of HTLV-1 is determined in large part by properties of the core particle and by the efficiency of postentry processes.

* Corresponding author. Mailing address: Basic Research Laboratory, Bldg. 567, NCI-Frederick, Frederick, MD 21702-1201. Phone: (301) 846-5611. Fax: (301) 846-6863. E-mail: derse{at}ncifcrf.gov.

Journal of Virology, September 2001, p. 8461-8468, Vol. 75, No. 18
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.18.8461-8468.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Jouvenet, N., Neil, S. J. D., Zhadina, M., Zang, T., Kratovac, Z., Lee, Y., McNatt, M., Hatziioannou, T., Bieniasz, P. D. (2009). Broad-Spectrum Inhibition of Retroviral and Filoviral Particle Release by Tetherin. J. Virol. 83: 1837-1844 [Abstract] [Full Text]  
  • Liu, M., Yang, L., Zhang, L., Liu, B., Merling, R., Xia, Z., Giam, C.-Z. (2008). Human T-Cell Leukemia Virus Type 1 Infection Leads to Arrest in the G1 Phase of the Cell Cycle. J. Virol. 82: 8442-8455 [Abstract] [Full Text]  
  • Li, K., Zhang, S., Kronqvist, M., Wallin, M., Ekstrom, M., Derse, D., Garoff, H. (2008). Intersubunit Disulfide Isomerization Controls Membrane Fusion of Human T-Cell Leukemia Virus Env. J. Virol. 82: 7135-7143 [Abstract] [Full Text]  
  • Rulli, S. J. Jr., Mirro, J., Hill, S. A., Lloyd, P., Gorelick, R. J., Coffin, J. M., Derse, D., Rein, A. (2008). Interactions of Murine APOBEC3 and Human APOBEC3G with Murine Leukemia Viruses. J. Virol. 82: 6566-6575 [Abstract] [Full Text]  
  • Heidecker, G., Lloyd, P. A., Soheilian, F., Nagashima, K., Derse, D. (2007). The Role of WWP1-Gag Interaction and Gag Ubiquitination in Assembly and Release of Human T-Cell Leukemia Virus Type 1. J. Virol. 81: 9769-9777 [Abstract] [Full Text]  
  • Derse, D., Crise, B., Li, Y., Princler, G., Lum, N., Stewart, C., McGrath, C. F., Hughes, S. H., Munroe, D. J., Wu, X. (2007). Human T-Cell Leukemia Virus Type 1 Integration Target Sites in the Human Genome: Comparison with Those of Other Retroviruses. J. Virol. 81: 6731-6741 [Abstract] [Full Text]  
  • Chevalier, S. A., Walic, M., Calattini, S., Mallet, A., Prevost, M.-C., Gessain, A., Mahieux, R. (2007). Construction and Characterization of a Full-Length Infectious Simian T-Cell Lymphotropic Virus Type 3 Molecular Clone. J. Virol. 81: 6276-6285 [Abstract] [Full Text]  
  • Mitchell, M. S., Bodine, E. T., Hill, S., Princler, G., Lloyd, P., Mitsuya, H., Matsuoka, M., Derse, D. (2007). Phenotypic and Genotypic Comparisons of Human T-Cell Leukemia Virus Type 1 Reverse Transcriptases from Infected T-Cell Lines and Patient Samples. J. Virol. 81: 4422-4428 [Abstract] [Full Text]  
  • Derse, D., Hill, S. A., Princler, G., Lloyd, P., Heidecker, G. (2007). Resistance of human T cell leukemia virus type 1 to APOBEC3G restriction is mediated by elements in nucleocapsid. Proc. Natl. Acad. Sci. USA 104: 2915-2920 [Abstract] [Full Text]  
  • Mazurov, D., Heidecker, G., Derse, D. (2007). The Inner Loop of Tetraspanins CD82 and CD81 Mediates Interactions with Human T Cell Lymphotrophic Virus Type 1 Gag Protein. J. Biol. Chem. 282: 3896-3903 [Abstract] [Full Text]  
  • Wencker, M., Sausse, C., Derse, D., Gazzolo, L., Duc Dodon, M. (2007). Human T-Cell Leukemia Virus Type 1 Tax Protein Down-Regulates Pre-T-Cell Receptor Alpha Gene Transcription in Human Immature Thymocytes. J. Virol. 81: 301-308 [Abstract] [Full Text]  
  • Miyazato, P., Yasunaga, J.-i., Taniguchi, Y., Koyanagi, Y., Mitsuya, H., Matsuoka, M. (2006). De Novo Human T-Cell Leukemia Virus Type 1 Infection of Human Lymphocytes in NOD-SCID, Common {gamma}-Chain Knockout Mice. J. Virol. 80: 10683-10691 [Abstract] [Full Text]  
  • Mitchell, M. S., Tozser, J., Princler, G., Lloyd, P. A., Auth, A., Derse, D. (2006). Synthesis, Processing, and Composition of the Virion-associated HTLV-1 Reverse Transcriptase. J. Biol. Chem. 281: 3964-3971 [Abstract] [Full Text]  
  • Jones, K. S., Akel, S., Petrow-Sadowski, C., Huang, Y., Bertolette, D. C., Ruscetti, F. W. (2005). Induction of Human T Cell Leukemia Virus Type I Receptors on Quiescent Naive T Lymphocytes by TGF-{beta}. J. Immunol. 174: 4262-4270 [Abstract] [Full Text]  
  • Heidecker, G., Lloyd, P. A., Fox, K., Nagashima, K., Derse, D. (2004). Late Assembly Motifs of Human T-Cell Leukemia Virus Type 1 and Their Relative Roles in Particle Release. J. Virol. 78: 6636-6648 [Abstract] [Full Text]  
  • Chung, H.-K., Young, H. A., Goon, P. K. C., Heidecker, G., Princler, G. L., Shimozato, O., Taylor, G. P., Bangham, C. R. M., Derse, D. (2003). Activation of interleukin-13 expression in T cells from HTLV-1-infected individuals and in chronically infected cell lines. Blood 102: 4130-4136 [Abstract] [Full Text]  
  • Igakura, T., Stinchcombe, J. C., Goon, P. K. C., Taylor, G. P., Weber, J. N., Griffiths, G. M., Tanaka, Y., Osame, M., Bangham, C. R. M. (2003). Spread of HTLV-I Between Lymphocytes by Virus-Induced Polarization of the Cytoskeleton. Science 299: 1713-1716 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»