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Journal of Virology, September 2001, p. 8434-8439, Vol. 75, No. 18
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.18.8434-8439.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Chimeric Plant Virus Particles as Immunogens for Inducing
Murine and Human Immune Responses against Human Immunodeficiency
Virus Type 1
Carla
Marusic,1
Paola
Rizza,2
Laura
Lattanzi,2
Camillo
Mancini,1
Massimo
Spada,2
Filippo
Belardelli,2
Eugenio
Benvenuto,1,* and
Imerio
Capone2,*
Divisione Biotecnologie e Agricoltura, ENEA,
00060 Rome,1 and Laboratorio di
Virologia, Istituto Superiore di Sanità, 00161 Rome,2 Italy
Received 23 April 2001/Accepted 12 June 2001
The high-yield expression of a neutralizing epitope from human
immunodeficiency virus type 1 (HIV-1) on the surface of a plant virus
and its immunogenicity are presented. The highly conserved ELDKWA
epitope from glycoprotein (gp) 41 was expressed as an N-terminal translational fusion with the potato virus X (PVX) coat protein. The
resulting chimeric virus particles (CVPs), purified and used to
immunize mice intraperitoneally or intranasally, were able to elicit
high levels of HIV-1-specific immunoglobulin G (IgG) and IgA
antibodies. Furthermore, the human immune response to CVPs was studied
with severe combined immunodeficient mice reconstituted with human
peripheral blood lymphocytes (hu-PBL-SCID). hu-PBL-SCID mice immunized
with CVP-pulsed autologous dendritic cells were able to mount a
specific human primary antibody response against the gp41-derived
epitope. Notably, sera from both normal and hu-PBL-SCID mice showed an
anti-HIV-1-neutralizing activity. Thus, PVX-based CVPs carrying
neutralizing epitopes can offer novel perspectives for the development
of effective vaccines against HIV and, more generally, for the design
of new vaccination strategies in humans.
*
Corresponding author. Mailing address for Eugenio
Benvenuto: ENEA, Divisione Biotecnologie e Agricoltura, C.R. Casaccia,
00060 Rome, Italy. Phone: 39-06-30486347. Fax: 39-06-30484741. E-mail: benvenutoe{at}casaccia.enea.it. Mailing address for Imerio
Capone: Istituto Superiore di Sanità, Laboratorio di Virologia,
00161 Rome, Italy. Phone: 39-6-49903294. Fax: 39-6-49902097. E-mail: capone{at}iss.it.
Journal of Virology, September 2001, p. 8434-8439, Vol. 75, No. 18
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.18.8434-8439.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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