Latent Antigen Vaccination in a Model Gammaherpesvirus Infection

doi:10.1128/JVI.75.17.8283-8288.2001

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Journal of Virology, September 2001, p. 8283-8288, Vol. 75, No. 17
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.17.8283-8288.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Latent Antigen Vaccination in a Model Gammaherpesvirus Infection

Edward J. Usherwood,¹^, Kimberley A. Ward,¹^, Marcia A. Blackman,¹ James P. Stewart,² and David L. Woodland¹^,^*

The Trudeau Institute, Saranac Lake, New York 12983,¹ and The Laboratory for Clinical and Molecular Virology, Department of Veterinary Pathology, The University of Edinburgh, Edinburgh EH9 1QH, United Kingdom²

Received 3 April 2001/Accepted 4 June 2001

Vaccines that can reduce the load of latent gammaherpesvirus infections are eagerly sought. One attractive strategy is vaccinationagainst latency-associated proteins, which may increase the efficiencywith which T cells recognize and eliminate latently infected cells.However, due to the lack of tractable animal model systems, theeffect of latent-antigen vaccination on gammaherpesvirus latencyis not known. Here we use the murine gammaherpesvirus model toinvestigate the impact of vaccination with the latency-associatedM2 antigen. As expected, vaccination had no effect on the acutelung infection. However, there was a significant reduction inthe load of latently infected cells in the initial stages of thelatent infection, when M2 is expressed. These data show for thefirst time that latent-antigen vaccination can reduce the levelof latency in vivo and suggest that vaccination strategies involvingother latent antigens may ultimately be successfully used to reducethe long-term latentinfection.

^* Corresponding author. Mailing address: The Trudeau Institute, 100 Algonquin Ave., Saranac Lake, NY 12983. Phone: (518) 891-3080,ext. 314. Fax: (518) 891-5126. E-mail: dwoodland{at}trudeauinstitute.org.

Present address: Department of Microbiology and Immunology, Dartmouth Medical School, Lebanon, N.H.

Present address: University of Vermont, Department of Pathology, Burlington, VT05405.

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