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Journal of Virology, September 2001, p. 8016-8020, Vol. 75, No. 17
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.17.8016-8020.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Antibody-Directed Targeting of Retroviral Vectors via Cell Surface Antigens

Kouki Morizono,1 Gregory Bristol,2 Yi-ming Xie,1 Sam Kam-Pun Kung,1 and Irvin S. Y. Chen1,2,3,*

Department of Microbiology, Immunology and Molecular Genetics1 Department of Medicine,2 and UCLA AIDS Institute,3 UCLA School of Medicine, Los Angeles, California 90095

Received 19 January 2001/Accepted 4 June 2001

Targeted stable transduction of specific cells is a highly desirable goal for gene therapy applications. We report an efficient and broadly applicable approach for targeting retroviral vectors to specific cells. We find that the envelope of the alphavirus Sindbis virus can pseudotype human immunodeficiency virus type 1- and murine leukemia virus-based retroviral vectors. When modified to contain the Fc-binding domain of protein A, this envelope gives a significant enhancement in specificity in combination with antibodies specific for HLA and CD4 relative to that without antibody. Unlike previous targeting strategies for retroviral transduction, the virus titers are relatively high and stable and can be further increased by ultracentrifugation. This study provides proof of principle for a targeting strategy that would be generally useful for many gene therapy applications.

* Corresponding author. Mailing address: UCLA AIDS Institute, 10833 Le Conte Ave., 11-934 Factor Bldg., Los Angeles, CA 90095. Phone: (310) 825-4793. Fax: (310) 794-7682. E-mail: rtaweesu{at}ucla.edu.

Journal of Virology, September 2001, p. 8016-8020, Vol. 75, No. 17
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.17.8016-8020.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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