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Journal of Virology, August 2001, p. 7778-7784, Vol. 75, No. 16
Centro de Biología Molecular
(CSIC-UAM), Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain
Received 26 February 2001/Accepted 9 May 2001
A Sindbis virus (SV) variant with a 6K gene partially
deleted has been obtained. This SV Del6K virus is defective in the
proteolytic processing of virus glycoprotein precursor, transport of
glycoproteins to the plasma membrane, and plaque phenotype. A revertant
virus (SV Del6K-revQ21L) containing a point mutation in the deleted 6K
gene was isolated and characterized. SV Del6K-revQ21L has corrected the
defects of proteolytic processing and transport of virus glycoproteins to the plasma membrane, but it still remains attenuated compared to
wild-type (wt) SV, exhibiting defects in virus budding. Neither mutant
nor revertant viruses are complemented by the coexpression in
trans of a wt SV 6K gene.
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.16.7778-7784.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Sindbis Virus Variant with a Deletion in the 6K
Gene Shows Defects in Glycoprotein Processing and Trafficking: Lack of
Complementation by a Wild-Type 6K Gene in
trans
*
Corresponding author. Mailing address: Centro de
Biología Molecular (CSIC-UAM), Universidad Autónoma
de Madrid, Cantoblanco, 28049 Madrid, Spain. Phone:
34-91-397-8450. Fax: 34-91-397-4799. E-mail:
lcarrasco{at}cbm.uam.es.
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