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Journal of Virology, August 2001, p. 7727-7731, Vol. 75, No. 16
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.16.7727-7731.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

DNA-Induced Structural Changes in the Papillomavirus Capsid

Claudia Fligge, Frank Schäfer, Hans-Christoph Selinka, Cornelia Sapp, and Martin Sapp*

Institute for Medical Microbiology and Hygiene, University of Mainz, D-55101 Mainz, Germany

Received 27 March 2001/Accepted 10 May 2001

Human papillomavirus capsid assembly requires intercapsomeric disulfide bonds between molecules of the major capsid protein L1. Virions isolated from naturally occurring lesions have a higher degree of cross-linking than virus-like particles (VLPs), which have been generated in eukaryotic expression systems. Here we show that DNA encapsidation into VLPs leads to increased cross-linking between L1 molecules comparable to that seen in virions. A higher trypsin resistance, indicating a tighter association of capsomeres through DNA interaction, accompanies this structural change.

* Corresponding author. Mailing address: Institute for Medical Microbiology and Hygiene, Johannes-Gutenberg-Universität Mainz, Hochhaus am Augustusplatz, D-55101 Mainz, Germany. Phone: 49-6131-393-0211. Fax: 49-6131-393-2359. E-mail: sapp{at}mail.uni-mainz.de.

Journal of Virology, August 2001, p. 7727-7731, Vol. 75, No. 16
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.16.7727-7731.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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