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Journal of Virology, August 2001, p. 7727-7731, Vol. 75, No. 16
Institute for Medical Microbiology and
Hygiene, University of Mainz, D-55101 Mainz, Germany
Received 27 March 2001/Accepted 10 May 2001
Human papillomavirus capsid assembly requires intercapsomeric
disulfide bonds between molecules of the major capsid protein L1.
Virions isolated from naturally occurring lesions have a higher degree
of cross-linking than virus-like particles (VLPs), which have been
generated in eukaryotic expression systems. Here we show that DNA
encapsidation into VLPs leads to increased cross-linking between L1
molecules comparable to that seen in virions. A higher trypsin
resistance, indicating a tighter association of capsomeres through DNA
interaction, accompanies this structural change.
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.16.7727-7731.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
DNA-Induced Structural Changes in the
Papillomavirus Capsid
*
Corresponding author. Mailing address: Institute for
Medical Microbiology and Hygiene, Johannes-Gutenberg-Universität
Mainz, Hochhaus am Augustusplatz, D-55101 Mainz, Germany. Phone:
49-6131-393-0211. Fax: 49-6131-393-2359. E-mail:
sapp{at}mail.uni-mainz.de.
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