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Journal of Virology, August 2001, p. 7592-7601, Vol. 75, No. 16
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.16.7592-7601.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Requirement for Uracil-DNA Glycosylase during the
Transition to Late-Phase Cytomegalovirus DNA Replication
Charmain Tan
Courcelle,1,
Justin
Courcelle,2
Mark N.
Prichard,3 and
Edward
S.
Mocarski1,*
Department of Microbiology and Immunology,
Stanford University, Stanford, California
943051; Department of Biological
Sciences, Mississippi State University, Mississippi State, Mississippi
397622; and Aviron Inc., Mountain
View, California 940863
Received 31 January 2001/Accepted 4 May 2001
Cytomegalovirus gene UL114, a homolog of mammalian uracil-DNA
glycosylase (UNG), is required for efficient viral DNA replication. In
quiescent fibroblasts, UNG mutant virus replication is delayed for
48 h and follows the virus-induced expression of cellular UNG. In
contrast, mutant virus replication proceeds without delay in actively
growing fibroblasts that express host cell UNG. In the absence of viral
or host cell UNG expression, mutant virus fails to proceed to
late-phase DNA replication, characterized by rapid DNA amplification.
The data suggest that uracil incorporated early during wild-type viral
DNA replication must be removed by virus or host UNG prior to
late-phase amplification and encapsidation into progeny virions. The
process of uracil incorporation and excision may introduce strand
breaks to facilitate the transition from early-phase replication to
late-phase amplification.
*
Corresponding author. Mailing address: Department of
Microbiology and Immunology, Stanford University, Stanford, CA
94305-5124. Phone: (650) 723-6435. Fax: (650) 723-1606. E-mail:
mocarski{at}stanford.edu.

Present address: Office of Agricultural Communications, Mississippi
State University, Mississippi State, MS
39762.
Journal of Virology, August 2001, p. 7592-7601, Vol. 75, No. 16
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.16.7592-7601.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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