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Journal of Virology, August 2001, p. 7215-7218, Vol. 75, No. 15
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.15.7215-7218.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Deletion Mapping of the Encephalomyocarditis Virus Primary Cleavage Site

Harry Hahndagger and Ann C. Palmenberg*

Institute for Molecular Virology and Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706

Received 28 December 2000/Accepted 25 April 2001

The cotranslational, primary self-cleavage reaction of cardiovirus polyprotein relies on a highly conserved, short segment of amino acids at the 2A-2B protein boundary. The amino terminus of the required element for encephalomyocarditis virus has now been mapped to include Tyr126 of the 2A protein, the 18th amino acid before the cleavage site.

* Corresponding author. Mailing address: Department of Biochemistry, 433 Babcock Dr., Madison, WI 53706. Phone: (608) 262-7519. Fax: (608) 262-6690. E-mail: acpalmen{at}facstaff.wisc.edu.

dagger Present address: 2440 Barrington Ave. #309, Los Angeles, CA 90064.

Journal of Virology, August 2001, p. 7215-7218, Vol. 75, No. 15
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.15.7215-7218.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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