E Box-Dependent Activation of Telomerase by Human Papillomavirus Type 16 E6 Does Not Require Induction of c-myc

doi:10.1128/JVI.75.15.7198-7201.2001

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Journal of Virology, August 2001, p. 7198-7201, Vol. 75, No. 15
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.15.7198-7201.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

E Box-Dependent Activation of Telomerase by Human Papillomavirus Type 16 E6 Does Not Require Induction of c-myc

Lindy Gewin¹^,² and Denise A. Galloway¹^,^*

Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109,¹, and Molecular and Cellular Biology Program, University of Washington, Seattle, Washington 98195²

Received 20 February 2001/Accepted 11 May 2001

Human papillomavirus type 16 (HPV-16) E6 activates telomerase specifically in epithelial cells. The oncogene c-myc has alsobeen shown to activate telomerase in several cell types. Herewe show that while both HPV-16 E6 and c-myc require intact E boxesto transactivate the hTERT promoter, E6 does not induce hTERTtranscription simply by inducing expression of c-myc. Moreover,hTERT transactivation by HPV-16 E6 correlates with its abilityto bind the cellular E6-associated protein (E6AP), suggestingthat E6 and E6AP may target a regulator of hTERTexpression.

^* Corresponding author. Mailing address: Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., Mailstop C1-015, Seattle,WA 98109. Phone: (206) 667-4500. Fax: (206) 667-5815. E-mail:dgallowa{at}fhcrc.org.

Journal of Virology, August 2001, p. 7198-7201, Vol. 75, No. 15
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.15.7198-7201.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

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