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Journal of Virology, August 2001, p. 7193-7197, Vol. 75, No. 15
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.15.7193-7197.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Context-Dependent Phenotype of a Human Immunodeficiency Virus Type 1 Nucleocapsid Mutation

Andrea Cimarellidagger and Jeremy Luban*

Departments of Microbiology and Medicine, College of Physicians and Surgeons, Columbia Univeirsity, New York, New York, 10032

Received 31 October 2000/Accepted 3 May 2001

The human immunodeficiency virus type 1 (HIV-1) nucleocapsid mutation R10A/K11A abolishes viral replication when present in proviral clone HIV-1HXB-2, but it was found to have minimal effect on replication of the closely related HIV-1NL4-3. Functional mapping demonstrated that a nonconservative amino acid change at nucleocapsid residue 24 (threonine in HIV-1HXB-2, isoleucine in HIV-1NL4-3) is the major determinant of the different R10A/K11A phenotypes in these two proviruses. Threonine-isoleucine exchanges appear to modify the R10A/K11A phenotype via effects on virion RNA-packaging efficiency. The improved packaging seen with hydrophobic isoleucine is consistent with solution structures localizing this residue to a hydrophobic pocket that contacts guanosine bases in viral genomic RNA stem-loops critical for packaging.

* Corresponding author. Mailing address: Departments of Microbiology and Medicine, Columbia University, College of Physicians and Surgeons, 701 W. 168th St., New York, NY 10032. Phone: (212) 305-8706. Fax: (212) 305-0333. E-mail: JL45{at}columbia.edu.

dagger Present address: Ecole Normale Supérieure de Lyon, 69364 Lyon, France.

Journal of Virology, August 2001, p. 7193-7197, Vol. 75, No. 15
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.15.7193-7197.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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Copyright © 2001 by the American Society for Microbiology. All rights reserved.