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Journal of Virology, August 2001, p. 7078-7085, Vol. 75, No. 15
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.15.7078-7085.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

N-Terminal Domain of Borna Disease Virus G (p56) Protein Is Sufficient for Virus Receptor Recognition and Cell Entry†

Mar Perez,1 Michiko Watanabe,1,Dagger Michael A. Whitt,2 and Juan Carlos de la Torre1,*

Department of Neuropharmacology, Division of Virology, The Scripps Research Institute, La Jolla, California 92037,1 and Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, Tennessee 381632

Received 22 January 2001/Accepted 30 April 2001

Borna disease virus (BDV) surface glycoprotein (GP) (p56) has a predicted molecular mass of 56 kDa. Due to extensive posttranslational glycosylation the protein migrates as a polypeptide of 84 kDa (gp84). The processing of gp84 by the cellular protease furin generates gp43, which corresponds to the C-terminal part of gp84. Both gp84 and gp43 have been implicated in viral entry involving receptor-mediated endocytosis and pH-dependent fusion. We have investigated the domains of BDV p56 involved in virus entry. For this, we used a pseudotype approach based on a recently developed recombinant vesicular stomatitis virus (VSV) in which the gene for green fluorescent protein was substituted for the VSV G protein gene (VSVDelta G*). Complementation of VSVDelta G* with BDV p56 resulted in infectious VSVDelta G* pseudotypes that contained both BDV gp84 and gp43. BDV-VSV chimeric GPs that contained the N-terminal 244 amino acids of BDV p56 and amino acids 421 to 511 of VSV G protein were efficiently incorporated into VSVDelta G* particles, and the resulting pseudotype virions were neutralized by BDV-specific antiserum. These findings indicate that the N-terminal part of BDV p56 is sufficient for receptor recognition and virus entry.

* Corresponding author. Mailing address: The Scripps Research Institute, IMM6, 10550 N. Torrey Pines Rd., La Jolla, CA 92037. Phone: (858) 784-9462. Fax: (858) 784-9981. E-mail: juanct{at}scripps.edu.

dagger Publication 13774-NP from The Scripps Research Institute.

Dagger Present address: Department of Microbiology, Akita University School of Medicine, Akita, Japan.

Journal of Virology, August 2001, p. 7078-7085, Vol. 75, No. 15
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.15.7078-7085.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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