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Journal of Virology, August 2001, p. 6914-6922, Vol. 75, No. 15
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.15.6914-6922.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Characterization of a Late Entry Event in the Replication Cycle of Human Immunodeficiency Virus Type 2

Áine McKnight,1,* David J. Griffiths,1 Matthias Dittmar,2 Paul Clapham,3 and Elaine Thomas1

Wohl Virion Centre, Windeyer Institute of Medical Sciences, University College London, London W1T 4JF, United Kingdom1; Hygiene-Institut, Abteilung Virologie, D-69120 Heidelberg, Germany2; and University of Massachusetts Medical School, Worcester, Massachusetts 016053

Received 14 August 2000/Accepted 8 May 2001

Certain human cell lines and primary macrophage cultures are restricted to infection by some primary isolates of human immunodeficiency virus type 2 (HIV-2), although early steps of the viral life cycle such as fusion at the plasma membrane and reverse transcription are fully supported. The late postintegration events, transcription, translation, assembly, budding, and maturation into infectious virions are functional in restrictive cells. Apart from primary macrophages, the restrictive cell types are actively dividing, and nuclear import of preintegration complexes (PICs) is not required for infection. We therefore postulate that the PICs are trapped in a cellular compartment, preventing subsequent steps in the replication cycle that lead to integration of the provirus. To test this we showed that HIV-2 particles pseudotyped with vesicular stomatitis virus envelope G protein, which delivers HIV into an endocytic compartment, could overcome the block to infection. We suggest that delivery of the viral core into an appropriate cellular compartment is a critical step during the entry process of HIV.

* Corresponding author. Mailing address: Wohl Virion Centre, Windeyer Institute of Medical Sciences, UCL, 46 Cleveland St., London W1T 4JF, United Kingdom. Phone: 44 0 20 7679 9581. Fax: 44 0 20 7679 9555. E-mail: a.mcknight{at}ucl.ac.uk.

Journal of Virology, August 2001, p. 6914-6922, Vol. 75, No. 15
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.15.6914-6922.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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