Octamer-Binding Sequence Is a Key Element for the Autoregulation of Kaposi's Sarcoma-Associated Herpesvirus ORF50/Lyta Gene Expression

doi:10.1128/JVI.75.15.6894-6900.2001

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Journal of Virology, August 2001, p. 6894-6900, Vol. 75, No. 15
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.15.6894-6900.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Octamer-Binding Sequence Is a Key Element for the Autoregulation of Kaposi's Sarcoma-Associated Herpesvirus ORF50/Lyta Gene Expression

Shuhei Sakakibara,¹ Keiji Ueda,¹^,^* Jiguo Chen,¹ Toshiomi Okuno,² and Koichi Yamanishi¹

Department of Microbiology, Osaka University Medical School, Suita, Osaka 565-0871,¹ and Department of Microbiology, Hyogo Medical College, Nishinomiya, Hyogo 663-8501,² Japan

Received 2 January 2001/Accepted 4 May 2001

The expression of the Kaposi's sarcoma-associated herpesvirus (KSHV) open reading frame 50 (ORF50) protein, Lyta (lytic transactivator),marks the switch from latent KSHV infection to the lytic phase.ORF50/Lyta upregulates several target KSHV genes, such as K8 (K-bZip),K9 (vIRF1), and ORF57, finally leading to the production of matureviruses. The auto-upregulation of ORF50/Lyta is thought to bean important mechanism for efficient lytic viral replication.In this study, we focused on this autoregulation and identifiedthe promoter element required for it. An electrophoretic mobilityshift assay indicated that the octamer-binding protein 1 (Oct-1)bound to this element. Mutations in the octamer-binding motifresulted in refractoriness of the ORF50/Lyta promoter to transactivationby ORF50/Lyta, and Oct-1 expression enhanced this transactivation.These results suggest that the autoregulation of ORF50/Lyta ismediated by Oct-1.

^* Corresponding author. Mailing address: Department of Microbiology, Osaka University Medical School, 2-2 Yamada-oka, Suita,Osaka 565-0871, Japan. Phone: 81-6-6879-3321. Fax: 81-6-6879-3329.E-mail: kueda{at}micro.med.osaka-u.ac.jp.

Journal of Virology, August 2001, p. 6894-6900, Vol. 75, No. 15
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.15.6894-6900.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

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