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Journal of Virology, July 2001, p. 6714-6718, Vol. 75, No. 14
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.14.6714-6718.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Viral Protein U (Vpu)-Mediated Enhancement of Human Immunodeficiency Virus Type 1 Particle Release Depends on the Rate of Cellular Proliferation

Aparna Deora and Lee Ratner*

Departments of Medicine, Pathology, and Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110

Received 20 November 2000/Accepted 13 April 2001

Viral protein U (Vpu) is a 17-kDa phosphoprotein that enhances the release of viral particles from human immunodeficiency virus type 1-infected cells. This study shows that the effect of Vpu on efficient particle release depends on the rate of cell proliferation. Cells arrested by contact inhibition, chemical arresting agents, or terminal differentiation (i.e., macrophages) all exhibited a striking dependence on Vpu for efficient particle release, as shown by examination of particle production from transfections with full-length clones, infections, and the vaccinia virus expression system. In contrast, actively proliferating cells did not exhibit enhanced particle release with Vpu expression. This study demonstrates the necessity of Vpu for efficient viral particle release from quiescent cells.

* Corresponding author. Mailing address: Box 8069, 660 S. Euclid, Ave., Washington University School of Medicine, St. Louis, MO 63110. Phone: (314) 362-8836. Fax: (314) 747-2797. E-mail: LRATNER{at}imgate.wustl.edu.

Journal of Virology, July 2001, p. 6714-6718, Vol. 75, No. 14
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.14.6714-6718.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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J. Bacteriol. Mol. Cell. Biol. Microbiol. Mol. Biol. Rev.
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Copyright © 2001 by the American Society for Microbiology. All rights reserved.