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Journal of Virology, July 2001, p. 6609-6614, Vol. 75, No. 14
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.14.6609-6614.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Recessive Resistance in Pisum sativum and Potyvirus Pathotype Resolved in a Gene-for-Cistron Correspondence between Host and Virus

I. Elisabeth Johansen,* Ole Søgaard Lund, Charlotte K. Hjulsager, and Jesper Laursendagger

Biotechnology Group, Danish Institute of Agricultural Sciences, DK-1871 Frederiksberg C, Denmark

Received 29 January 2001/Accepted 23 April 2001

Pea seed-borne mosaic potyvirus (PSbMV) isolates are divided into pathotypes P-1, P-2, and P-4 according to their infection profile on a panel of Pisum sativum lines. P. sativum PI 269818 is resistant to P-1 and P-2 isolates and is susceptible to P-4 isolates. Resistance to P-1 is inherited as a single recessive gene, denoted sbm-1, and the pathogenicity determinant has previously been mapped to the virus-coded protein VPg. In the cultivar Bonneville, a second recessive gene, sbm-2, confers specific resistance to P-2. By exchanging cistrons between a P-2 and a P-4 isolate, the P3-6k1 cistron was identified as the PSbMV host-specific pathogenicity determinant on Bonneville. Exchange of P3-6k1 did not affect infection on PI 269818, and infection of Bonneville was not altered by substitution of the VPg cistron, indicating that P3-6k1 and VPg are independent determinants of pathotype-specific infectivity. On PI 269818 the pathogenicity determinant of both P-1 and P-2 mapped to the N terminus of VPg. This suggests that VPg from the P-1 and P-2 isolates are functionally similar on this host and that resistance to P-1 and P-2 in PI 269818 may operate by the same mechanism. Identification of VPg-sbm-1 and P3-6k1-sbm-2 as independent pairs of genetic interactors between PSbMV and P. sativum provides a simple explanation of the three known pathotypes of PSbMV. Furthermore, analysis of beta -glucuronidase-tagged P-2 virus indicated that sbm-2 resistance affected an early step in infection, implying that the P3-6k1 region plays a critical role in potyvirus replication or cell-to-cell movement.

* Corresponding author. Mailing address: Danish Institute of Agricultural Sciences, Biotechnology Group, Thorvaldsensvej 40.1, DK-1871 Frederiksberg C, Denmark. Phone: 45 3528 2584. Fax: 45 3528 2589. E-mail: e.johansen{at}dias.kvl.dk.

dagger Present address: DAKO A/S, DK-2600 Glostrup, Denmark.

Journal of Virology, July 2001, p. 6609-6614, Vol. 75, No. 14
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.14.6609-6614.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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