This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dirks, C.
Right arrow Articles by Miller, A. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dirks, C.
Right arrow Articles by Miller, A. D.

 Previous Article  |  Next Article 

Journal of Virology, July 2001, p. 6375-6383, Vol. 75, No. 14
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.14.6375-6383.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Many Nonmammalian Cells Exhibit Postentry Blocks to Transduction by Gammaretroviruses Pseudotyped with Various Viral Envelopes, Including Vesicular Stomatitis Virus G Glycoprotein

Clarissa Dirks1,2 and A. Dusty Miller2,3,*

Molecular and Cellular Biology Program1 and Division of Human Biology,2 Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024, and Department of Pathology, University of Washington, Seattle, Washington 981953

Received 27 December 2000/Accepted 13 April 2001

Previous studies have suggested that Moloney murine leukemia virus (MoMLV)-based vectors pseudotyped with the vesicular stomatitis virus G glycoprotein (VSV-G) have extensive ability to transduce nonmammalian cells. However, we have identified multiple cell lines from fish (FHM), mosquitoes (Mos-55), moths (Sf9 and High-5), flies (S2), and frogs (XPK2) that are not efficiently transduced by MoMLV-based vectors pseudotyped with many different viral envelope proteins, including VSV-G, while the same vectors are functional in these cells following transfection. A comparison of MoMLV-based vector transduction in mammalian and nonmammalian cells shows that the nonmammalian cells exhibit blocks at either entry, reverse transcription, or integration. Additionally, VSV-G-pseudotyped MoMLV-based vector transduction is attenuated in the zebrafish cell line ZF4 at entry and/or reverse transcription, whereas other transduction processes are unaffected. We show that the variation of transduction by MoMLV-based vectors in mammalian and nonmammalian cells is not due to differences in culture conditions or cell division rate but is likely the result of divergence in cellular factors required for retroviral transduction.

* Corresponding author. Mailing address: Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., Room C2-105, Seattle, WA 98109-1024. Phone: (206) 667-2890. Fax: (206) 667-6523. E-mail: dmiller{at}fhcrc.org.

Journal of Virology, July 2001, p. 6375-6383, Vol. 75, No. 14
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.14.6375-6383.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kines, K. J., Morales, M. E., Mann, V. H., Gobert, G. N., Brindley, P. J. (2008). Integration of reporter transgenes into Schistosoma mansoni chromosomes mediated by pseudotyped murine leukemia virus. FASEB J. 22: 2936-2948 [Abstract] [Full Text]  
  • Coil, D. A., Miller, A. D. (2005). Enhancement of Enveloped Virus Entry by Phosphatidylserine. J. Virol. 79: 11496-11500 [Abstract] [Full Text]  
  • Kolokoltsov, A. A., Weaver, S. C., Davey, R. A. (2005). Efficient Functional Pseudotyping of Oncoretroviral and Lentiviral Vectors by Venezuelan Equine Encephalitis Virus Envelope Proteins. J. Virol. 79: 756-763 [Abstract] [Full Text]  
  • Coil, D. A., Miller, A. D. (2004). Phosphatidylserine Is Not the Cell Surface Receptor for Vesicular Stomatitis Virus. J. Virol. 78: 10920-10926 [Abstract] [Full Text]  
  • Jones, K. S., Nath, M., Petrow-Sadowski, C., Baines, A. C., Dambach, M., Huang, Y., Ruscetti, F. W. (2002). Similar Regulation of Cell Surface Human T-Cell Leukemia Virus Type 1 (HTLV-1) Surface Binding Proteins in Cells Highly and Poorly Transduced by HTLV-1-Pseudotyped Virions. J. Virol. 76: 12723-12734 [Abstract] [Full Text]  
  • Kang, Y., Stein, C. S., Heth, J. A., Sinn, P. L., Penisten, A. K., Staber, P. D., Ratliff, K. L., Shen, H., Barker, C. K., Martins, I., Sharkey, C. M., Sanders, D. A., McCray, P. B. Jr., Davidson, B. L. (2002). In Vivo Gene Transfer Using a Nonprimate Lentiviral Vector Pseudotyped with Ross River Virus Glycoproteins. J. Virol. 76: 9378-9388 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»