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Journal of Virology, July 2001, p. 6265-6272, Vol. 75, No. 14
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.14.6265-6272.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Fidelity of Leader and Trailer Sequence Usage by the Respiratory Syncytial Virus and Avian Pneumovirus Replication Complexes

Anthony C. Marriott,* Joanne M. Smith, and Andrew J. Easton

Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom

Received 16 January 2001/Accepted 17 April 2001

The specificity of usage of promoters for replication and transcription by the pneumoviruses human respiratory syncytial virus (HRSV) and avian pneumovirus (APV) was studied using minigenomes containing a reporter gene. When infectious HRSV or APV was used as helper virus, replication could occur only if both the leader and trailer regions (containing the replicative and transcriptional promoters) were derived from the helper virus. In contrast, when the HRSV replication complex was supplied from cDNA plasmids, a minigenome containing either the APV leader or trailer was recognized and substantial levels of replication and transcription occurred. These data suggest that in pneumovirus-infected cells, helper virus functions can discriminate between genomes on the basis of the terminal sequences and that there is an association between the leader and trailer required for productive replication. This association is required only in virus-infected cells, not when replication and transcription are mediated by plasmid-directed expression of the component proteins required for replication and transcription. The possible implications of this are discussed.

* Corresponding author. Mailing address: Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom. Phone: (44) 24 7652 3565. Fax: (44) 24 7652 3701. E-mail: a.c.marriott{at}warwick.ac.uk.

Journal of Virology, July 2001, p. 6265-6272, Vol. 75, No. 14
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.14.6265-6272.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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