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Journal of Virology, July 2001, p. 6223-6227, Vol. 75, No. 13
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.13.6223-6227.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Novel Baculovirus Expression Vectors That Provide Sialylation of Recombinant Glycoproteins in Lepidopteran Insect Cells

Donald L. Jarvis,* Dale Howe, and Jared J. Aumiller

Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071-3944

Received 27 December 2000/Accepted 9 April 2001

This report describes novel baculovirus vectors designed to express mammalian beta 1,4-galactosyltransferase and alpha 2,6-sialyltransferase genes at early times after infection. Sf9 cells infected with these viral vectors, unlike cells infected with a wild-type baculovirus, produced a sialylated viral glycoprotein during the late phase of infection. Thus, the two mammalian glycosyltransferases encoded by these viral vectors are necessary and sufficient for sialylation of a foreign glycoprotein in insect cells under the conditions used in this study. While some of the new baculovirus vectors described in this study produced less, one produced wild-type levels of infectious budded virus progeny.

* Corresponding author. Mailing address: Department of Molecular Biology, University of Wyoming, P.O. Box 3944, Laramie, WY 82071-3944. Phone: (307) 766-4282. Fax: (307) 766-5098. E-mail: DLJarvis{at}uwyo.edu.

Journal of Virology, July 2001, p. 6223-6227, Vol. 75, No. 13
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.13.6223-6227.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Aumiller, J. J., Hollister, J. R., Jarvis, D. L. (2003). A transgenic insect cell line engineered to produce CMP-sialic acid and sialylated glycoproteins. Glycobiology 13: 497-507 [Abstract] [Full Text]  
  • Hollister, J., Conradt, H., Jarvis, D. L. (2003). Evidence for a sialic acid salvaging pathway in lepidopteran insect cells. Glycobiology 13: 487-495 [Abstract] [Full Text]  
  • Watanabe, S., Kokuho, T., Takahashi, H., Takahashi, M., Kubota, T., Inumaru, S. (2002). Sialylation of N-Glycans on the Recombinant Proteins Expressed by a Baculovirus-Insect Cell System under beta -N-Acetylglucosaminidase Inhibition. J. Biol. Chem. 277: 5090-5093 [Abstract] [Full Text]  


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