This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by LaPierre, L. A.
Right arrow Articles by Biegalke, B. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by LaPierre, L. A.
Right arrow Articles by Biegalke, B. J.

 Previous Article  |  Next Article 

Journal of Virology, July 2001, p. 6062-6069, Vol. 75, No. 13
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.13.6062-6069.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of a Novel Transcriptional Repressor Encoded by Human Cytomegalovirus

Lorie A. LaPierre1,dagger and Bonita J. Biegalke1,2,*

Department of Biomedical Sciences, College of Osteopathic Medicine,1 and Molecular and Cellular Biology Program,2 Ohio University, Athens, Ohio 45701

Received 30 January 2001/Accepted 30 March 2001

The expression of human cytomegalovirus (HCMV) genes during viral replication is precisely regulated, with the interactions of both transcriptional activators and repressors determining the level of gene expression. One gene of HCMV, the US3 gene, is transcriptionally repressed early in infection. Repression of US3 expression requires viral infection and protein synthesis and is mediated through a DNA sequence, the transcriptional repressive element. In this report, we identify the protein that represses US3 transcription as the product of the HCMV UL34 open reading frame. The protein encoded by UL34 (pUL34) binds to the US3 transcriptional repressive element in yeast and in vitro. pUL34 localizes to the nucleus and alone is sufficient for repression of US3 expression. The data presented here, along with earlier data (B. J. Biegalke, J. Virol. 72:5457-5463, 1998), suggests that pUL34 binding of the transcriptional repressive element prevents transcription initiation complex formation.

* Corresponding author. Mailing address: Department of Biomedical Sciences, 228 Irvine Hall, Ohio University, Athens, OH 45701. Phone: (740) 593-2377. Fax: (740) 597-2778. E-mail: biegalke{at}ohiou.edu.

dagger Present address: Department of Biological Sciences, Ohio University, Athens, OH 45701.

Journal of Virology, July 2001, p. 6062-6069, Vol. 75, No. 13
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.13.6062-6069.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Biegalke, B. J., Lester, E., Branda, A., Rana, R. (2004). Characterization of the Human Cytomegalovirus UL34 Gene. J. Virol. 78: 9579-9583 [Abstract] [Full Text]  
  • Murphy, E., Rigoutsos, I., Shibuya, T., Shenk, T. E. (2003). Reevaluation of human cytomegalovirus coding potential. Proc. Natl. Acad. Sci. USA 100: 13585-13590 [Abstract] [Full Text]  
  • Liu, Y., Biegalke, B. J. (2002). The Human Cytomegalovirus UL35 Gene Encodes Two Proteins with Different Functions. J. Virol. 76: 2460-2468 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»