This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mikloska, Z.
Right arrow Articles by Cunningham, A. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mikloska, Z.
Right arrow Articles by Cunningham, A. L.

 Previous Article  |  Next Article 

Journal of Virology, July 2001, p. 5958-5964, Vol. 75, No. 13
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.13.5958-5964.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Immature Monocyte-Derived Dendritic Cells Are Productively Infected with Herpes Simplex Virus Type 1

Zorka Mikloska,1,* Lidija Bosnjak,1,2 and Anthony L. Cunningham1

Centre for Virus Research, Westmead Millennium Institute and University of Sydney, Westmead,1 and School of Microbiology and Immunology, University of New South Wales, Kensington,2 Australia

Received 27 December 2000/Accepted 3 April 2001

Herpes simplex viruses (HSV) have developed several immunoevasive strategies. Here we demonstrate a novel mechanism by which HSV type 1 may interfere with the immune response through infection of immature dendritic cells (DC) and selective downmodulation of costimulatory molecules. In our study we show productive infection of immature monocyte-derived DC, which closely resemble sessile Langerhans cells, by sequential expression of immediate-early, early, and late viral proteins and of glycoprotein D mRNA, as well as production of infectious virus of moderate titers. Infection was cytopathic, with the progressive loss of 20 to 45% of cells from 24 to 48 h after infection, with no more than 80% of DC found to be infected. These results are in contrast to those of previous findings of nonpermissive or abortive infection of monocytes and mature monocyte-derived DC. Infection of immature DC also led to selective and asynchronous downregulation of CD1a, CD40, CD54 (ICAM-1) (12 h postinfection), CD80 (24 h postinfection), and CD86 (48 h postinfection) but not of CD11c or major histocompatibility complex class I and II molecules when compared to DC exposed to UV-inactivated virus. Thus, we propose that productive infection of epidermal Langerhans cells in vivo may lead to delayed activation of T cells, allowing more time for replication of HSV type 1 in epidermal cells.

* Corresponding author. Mailing address: Centre for Virus Research, Westmead Millennium Institute, P.O. Box 412, Westmead, NSW 2145, Australia. Phone: 61-2-9845-9115. Fax: 61-2-9845-9100. E-mail: zorka_mikloska{at}wmi.usyd.edu.au.

Journal of Virology, July 2001, p. 5958-5964, Vol. 75, No. 13
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.13.5958-5964.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kather, A., Raftery, M. J., Devi-Rao, G., Lippmann, J., Giese, T., Sandri-Goldin, R. M., Schonrich, G. (2010). Herpes Simplex Virus Type 1 (HSV-1)-Induced Apoptosis in Human Dendritic Cells as a Result of Downregulation of Cellular FLICE-Inhibitory Protein and Reduced Expression of HSV-1 Antiapoptotic Latency-Associated Transcript Sequences. J. Virol. 84: 1034-1046 [Abstract] [Full Text]  
  • Lin, Y.-W., Wang, S.-W., Tung, Y.-Y., Chen, S.-H. (2009). Enterovirus 71 Infection of Human Dendritic Cells. Exp Biol Med 234: 1166-1173 [Abstract] [Full Text]  
  • Jin, H., Ma, Y., Prabhakar, B. S., Feng, Z., Valyi-Nagy, T., Yan, Z., Verpooten, D., Zhang, C., Cao, Y., He, B. (2009). The {gamma}134.5 Protein of Herpes Simplex Virus 1 Is Required To Interfere with Dendritic Cell Maturation during Productive Infection. J. Virol. 83: 4984-4994 [Abstract] [Full Text]  
  • Conrady, C. D., Thapa, M., Wuest, T., Carr, D. J. J. (2009). Loss of Mandibular Lymph Node Integrity Is Associated with an Increase in Sensitivity to HSV-1 Infection in CD118-Deficient Mice. J. Immunol. 182: 3678-3687 [Abstract] [Full Text]  
  • Lee, H. K., Zamora, M., Linehan, M. M., Iijima, N., Gonzalez, D., Haberman, A., Iwasaki, A. (2009). Differential roles of migratory and resident DCs in T cell priming after mucosal or skin HSV-1 infection. JEM 206: 359-370 [Abstract] [Full Text]  
  • Donaghy, H., Bosnjak, L., Harman, A. N., Marsden, V., Tyring, S. K., Meng, T.-C., Cunningham, A. L. (2009). Role for Plasmacytoid Dendritic Cells in the Immune Control of Recurrent Human Herpes Simplex Virus Infection. J. Virol. 83: 1952-1961 [Abstract] [Full Text]  
  • Prestwich, R. J., Errington, F., Ilett, E. J., Morgan, R. S.M., Scott, K. J., Kottke, T., Thompson, J., Morrison, E. E., Harrington, K. J., Pandha, H. S., Selby, P. J., Vile, R. G., Melcher, A. A. (2008). Tumor Infection by Oncolytic Reovirus Primes Adaptive Antitumor Immunity. Clin. Cancer Res. 14: 7358-7366 [Abstract] [Full Text]  
  • Dejnirattisai, W., Duangchinda, T., Lin, C.-L. S., Vasanawathana, S., Jones, M., Jacobs, M., Malasit, P., Xu, X.-n., Screaton, G., Mongkolsapaya, J. (2008). A Complex Interplay among Virus, Dendritic Cells, T Cells, and Cytokines in Dengue Virus Infections. J. Immunol. 181: 5865-5874 [Abstract] [Full Text]  
  • de Jong, M. A. W. P., de Witte, L., Bolmstedt, A., van Kooyk, Y., Geijtenbeek, T. B. H. (2008). Dendritic cells mediate herpes simplex virus infection and transmission through the C-type lectin DC-SIGN. J. Gen. Virol. 89: 2398-2409 [Abstract] [Full Text]  
  • Kummer, M., Turza, N. M., Muhl-Zurbes, P., Lechmann, M., Boutell, C., Coffin, R. S., Everett, R. D., Steinkasserer, A., Prechtel, A. T. (2007). Herpes Simplex Virus Type 1 Induces CD83 Degradation in Mature Dendritic Cells with Immediate-Early Kinetics via the Cellular Proteasome. J. Virol. 81: 6326-6338 [Abstract] [Full Text]  
  • Harman, A. N., Wilkinson, J., Bye, C. R., Bosnjak, L., Stern, J. L., Nicholle, M., Lai, J., Cunningham, A. L. (2006). HIV Induces Maturation of Monocyte-Derived Dendritic Cells and Langerhans Cells. J. Immunol. 177: 7103-7113 [Abstract] [Full Text]  
  • Trapp, S., Turville, S. G., Robbiani, M. (2006). Slamming the door on unwanted guests: why preemptive strikes at the mucosa may be the best strategy against HIV. J. Leukoc. Biol. 80: 1076-1083 [Full Text]  
  • Raftery, M. J., Winau, F., Kaufmann, S. H. E., Schaible, U. E., Schonrich, G. (2006). CD1 Antigen Presentation by Human Dendritic Cells as a Target for Herpes Simplex Virus Immune Evasion. J. Immunol. 177: 6207-6214 [Abstract] [Full Text]  
  • Suvas, S., Kim, B., Sarangi, P. P., Tone, M., Waldmann, H., Rouse, B. T. (2005). In Vivo Kinetics of GITR and GITR Ligand Expression and Their Functional Significance in Regulating Viral Immunopathology. J. Virol. 79: 11935-11942 [Abstract] [Full Text]  
  • Lauterbach, H., Ried, C., Epstein, A. L., Marconi, P., Brocker, T. (2005). Reduced immune responses after vaccination with a recombinant herpes simplex virus type 1 vector in the presence of antiviral immunity. J. Gen. Virol. 86: 2401-2410 [Abstract] [Full Text]  
  • Flano, E., Kayhan, B., Woodland, D. L., Blackman, M. A. (2005). Infection of Dendritic Cells by a {gamma}2-Herpesvirus Induces Functional Modulation. J. Immunol. 175: 3225-3234 [Abstract] [Full Text]  
  • Peretti, S., Shaw, A., Blanchard, J., Bohm, R., Morrow, G., Lifson, J. D., Gettie, A., Pope, M. (2005). Immunomodulatory effects of HSV-2 infection on immature macaque dendritic cells modify innate and adaptive responses. Blood 106: 1305-1313 [Abstract] [Full Text]  
  • Prechtel, A. T., Turza, N. M., Kobelt, D. J., Eisemann, J. I., Coffin, R. S., McGrath, Y., Hacker, C., Ju, X., Zenke, M., Steinkasserer, A. (2005). Infection of mature dendritic cells with herpes simplex virus type 1 dramatically reduces lymphoid chemokine-mediated migration. J. Gen. Virol. 86: 1645-1657 [Abstract] [Full Text]  
  • Gorantla, S., Santos, K., Meyer, V., Dewhurst, S., Bowers, W. J., Federoff, H. J., Gendelman, H. E., Poluektova, L. (2005). Human Dendritic Cells Transduced with Herpes Simplex Virus Amplicons Encoding Human Immunodeficiency Virus Type 1 (HIV-1) gp120 Elicit Adaptive Immune Responses from Human Cells Engrafted into NOD/SCID Mice and Confer Partial Protection against HIV-1 Challenge. J. Virol. 79: 2124-2132 [Abstract] [Full Text]  
  • Bosnjak, L., Miranda-Saksena, M., Koelle, D. M., Boadle, R. A., Jones, C. A., Cunningham, A. L. (2005). Herpes Simplex Virus Infection of Human Dendritic Cells Induces Apoptosis and Allows Cross-Presentation via Uninfected Dendritic Cells. J. Immunol. 174: 2220-2227 [Abstract] [Full Text]  
  • Pollara, G., Jones, M., Handley, M. E., Rajpopat, M., Kwan, A., Coffin, R. S., Foster, G., Chain, B., Katz, D. R. (2004). Herpes Simplex Virus Type-1-Induced Activation of Myeloid Dendritic Cells: The Roles of Virus Cell Interaction and Paracrine Type I IFN Secretion. J. Immunol. 173: 4108-4119 [Abstract] [Full Text]  
  • Gredmark, S., Soderberg-Naucler, C. (2003). Human Cytomegalovirus Inhibits Differentiation of Monocytes into Dendritic Cells with the Consequence of Depressed Immunological Functions. J. Virol. 77: 10943-10956 [Abstract] [Full Text]  
  • Samady, L., Costigliola, E., MacCormac, L., McGrath, Y., Cleverley, S., Lilley, C. E., Smith, J., Latchman, D. S., Chain, B., Coffin, R. S. (2003). Deletion of the Virion Host Shutoff Protein (vhs) from Herpes Simplex Virus (HSV) Relieves the Viral Block to Dendritic Cell Activation: Potential of vhs- HSV Vectors for Dendritic Cell-Mediated Immunotherapy. J. Virol. 77: 3768-3776 [Abstract] [Full Text]  
  • Zhao, X., Deak, E., Soderberg, K., Linehan, M., Spezzano, D., Zhu, J., Knipe, D. M., Iwasaki, A. (2003). Vaginal Submucosal Dendritic Cells, but Not Langerhans Cells, Induce Protective Th1 Responses to Herpes Simplex Virus-2. JEM 197: 153-162 [Abstract] [Full Text]  
  • Koelle, D. M., Corey, L. (2003). Recent Progress in Herpes Simplex Virus Immunobiology and Vaccine Research. Clin. Microbiol. Rev. 16: 96-113 [Abstract] [Full Text]  
  • Hocknell, P. K., Wiley, R. D., Wang, X., Evans, T. G., Bowers, W. J., Hanke, T., Federoff, H. J., Dewhurst, S. (2002). Expression of Human Immunodeficiency Virus Type 1 gp120 from Herpes Simplex Virus Type 1-Derived Amplicons Results in Potent, Specific, and Durable Cellular and Humoral Immune Responses. J. Virol. 76: 5565-5580 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»