Genomic Features of Intertypic Recombinant Sabin Poliovirus Strains Excreted by Primary Vaccinees

doi:10.1128/JVI.75.13.5740-5751.2001

This Article

	Full Text
	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Copyright Information
	Books from ASM Press
	MicrobeWorld

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Cuervo, N. S.
	Articles by Delpeyroux, F.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Cuervo, N. S.
	Articles by Delpeyroux, F.

Journal of Virology, July 2001, p. 5740-5751, Vol. 75, No. 13
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.13.5740-5751.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Genomic Features of Intertypic Recombinant Sabin Poliovirus Strains Excreted by Primary Vaccinees

Nancy Stella Cuervo,¹ Sophie Guillot,¹ Natalia Romanenkova,² Mariana Combiescu,³ André Aubert-Combiescu,³ Mohamed Seghier,⁴ Valérie Caro,¹ Radu Crainic,¹ and Francis Delpeyroux¹^,^*

Epidémiologie Moléculaire des Entérovirus, Institut Pasteur, Paris, France¹; Institut Pasteur de Saint-Pétersbourg, Saint Petersburg, Russia²; Institut Cantacuzino, Bucharest, Romania³; and Institut Pasteur d'Algérie, Algiers, Algeria⁴

Received 7 December 2000/Accepted 28 March 2001

The trivalent oral poliomyelitis vaccine (OPV) contains three different poliovirus serotypes. It use therefore creates particularlyfavorable conditions for mixed infection of gut cells, and indeedintertypic vaccine-derived recombinants (VdRec) have been frequentlyfound in patients with vaccine-associated paralytic poliomyelitis.Nevertheless, there have not been extensive searches for VdRecin healthy vaccinees following immunization with OPV. To determinethe incidence of VdRec and their excretion kinetics in primaryvaccinees, and to establish the general genomic features of thecorresponding recombinant genomes, we characterized poliovirusisolates excreted by vaccinees following primary immunizationwith OPV. Isolates were collected from 67 children 2 to 60 daysfollowing vaccination. Recombinant strains were identified bymultiple restriction fragment length polymorphism assays. Thelocalization of junction sites in recombinant genomes was alsodetermined. VdRec excreted by vaccinees were first detected 2to 4 days after vaccination. The highest rate of recombinantswas on day 14. The frequency of VdRec depends strongly on theserotype of the analyzed isolates (2, 53, and 79% of recombinantstrains in the last-excreted type 1, 2, and 3 isolates, respectively).Particular associations of genomic segments were preferred inthe recombinant genomes, and recombination junctions were foundin the genomic region encoding the nonstructural proteins. Recombinationjunctions generally clustered in particular subgenomic regionsthat were dependent on the serotype of the isolate and/or on theassociations of genomic segments in recombinants. Thus, VdRecare frequently excreted by vaccinees, and the poliovirus replicationmachinery requirements or selection factors appear to act in vivoto shape the features of the recombinantgenomes.

^* Corresponding author. Mailing address: Laboratoire d'Epidémiologie Moléculaire des Entérovirus, Institut Pasteur, 28 ruedu Dr Roux, 75724 Paris Cedex 15, France. Phone: (33) 1 40 6133 22. Fax: (33) 1 45 68 87 80. E-mail: delpeyro{at}pasteur.fr.

Journal of Virology, July 2001, p. 5740-5751, Vol. 75, No. 13
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.13.5740-5751.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

Bouslama, L., Nasri, D., Chollet, L., Belguith, K., Bourlet, T., Aouni, M., Pozzetto, B., Pillet, S. (2007). Natural Recombination Event within the Capsid Genomic Region Leading to a Chimeric Strain of Human Enterovirus B. J. Virol. 81: 8944-8952 [Abstract] [Full Text]
Simmonds, P. (2006). Recombination and Selection in the Evolution of Picornaviruses and Other Mammalian Positive-Stranded RNA Viruses. J. Virol. 80: 11124-11140 [Abstract] [Full Text]
Romanenkova, N. I., Guillot, S., Rozaeva, N. R., Crainic, R., Bichurina, M. A., Delpeyroux, F. (2006). Use of a Multiple Restriction Fragment Length Polymorphism Method for Detecting Vaccine-Derived Polioviruses in Clinical Samples. J. Clin. Microbiol. 44: 4077-4084 [Abstract] [Full Text]
Simmonds, P., Welch, J. (2006). Frequency and Dynamics of Recombination within Different Species of Human Enteroviruses. J. Virol. 80: 483-493 [Abstract] [Full Text]
Karakasiliotis, I., Paximadi, E., Markoulatos, P. (2005). Evolution of a rare vaccine-derived multirecombinant poliovirus. J. Gen. Virol. 86: 3137-3142 [Abstract] [Full Text]
Yang, C.-F., Chen, H.-Y., Jorba, J., Sun, H.-C., Yang, S.-J., Lee, H.-C., Huang, Y.-C., Lin, T.-Y., Chen, P.-J., Shimizu, H., Nishimura, Y., Utama, A., Pallansch, M., Miyamura, T., Kew, O., Yang, J.-Y. (2005). Intratypic Recombination among Lineages of Type 1 Vaccine-Derived Poliovirus Emerging during Chronic Infection of an Immunodeficient Patient. J. Virol. 79: 12623-12634 [Abstract] [Full Text]
Appel, N., Herian, U., Bartenschlager, R. (2005). Efficient Rescue of Hepatitis C Virus RNA Replication by trans-Complementation with Nonstructural Protein 5A. J. Virol. 79: 896-909 [Abstract] [Full Text]
Cherkasova, E. A., Yakovenko, M. L., Rezapkin, G. V., Korotkova, E. A., Ivanova, O. E., Eremeeva, T. P., Krasnoproshina, L. I., Romanenkova, N. I., Rozaeva, N. R., Sirota, L., Agol, V. I., Chumakov, K. M. (2005). Spread of Vaccine-Derived Poliovirus from a Paralytic Case in an Immunodeficient Child: an Insight into the Natural Evolution of Oral Polio Vaccine. J. Virol. 79: 1062-1070 [Abstract] [Full Text]
Papaventsis, D., Siafakas, N., Markoulatos, P., Papageorgiou, G. T., Kourtis, C., Chatzichristou, E., Economou, C., Levidiotou, S. (2005). Membrane Adsorption with Direct Cell Culture Combined with Reverse Transcription-PCR as a Fast Method for Identifying Enteroviruses from Sewage. Appl. Environ. Microbiol. 71: 72-79 [Abstract] [Full Text]
Rakoto-Andrianarivelo, M., Rousset, D., Razafindratsimandresy, R., Chevaliez, S., Guillot, S., Balanant, J., Delpeyroux, F. (2005). High Frequency of Human Enterovirus Species C Circulation in Madagascar. J. Clin. Microbiol. 43: 242-249 [Abstract] [Full Text]
Shimizu, H., Thorley, B., Paladin, F. J., Brussen, K. A., Stambos, V., Yuen, L., Utama, A., Tano, Y., Arita, M., Yoshida, H., Yoneyama, T., Benegas, A., Roesel, S., Pallansch, M., Kew, O., Miyamura, T. (2004). Circulation of Type 1 Vaccine-Derived Poliovirus in the Philippines in 2001. J. Virol. 78: 13512-13521 [Abstract] [Full Text]
Oberste, M. S., Penaranda, S., Pallansch, M. A. (2004). RNA Recombination Plays a Major Role in Genomic Change during Circulation of Coxsackie B Viruses. J. Virol. 78: 2948-2955 [Abstract] [Full Text]
Oberste, M. S., Maher, K., Pallansch, M. A. (2004). Evidence for Frequent Recombination within Species Human Enterovirus B Based on Complete Genomic Sequences of All Thirty-Seven Serotypes. J. Virol. 78: 855-867 [Abstract] [Full Text]
Costa-Mattioli, M., Napoli, A. D., Ferre, V., Billaudel, S., Perez-Bercoff, R., Cristina, J. (2003). Genetic variability of hepatitis A virus. J. Gen. Virol. 84: 3191-3201 [Abstract] [Full Text]
Lukashev, A. N., Lashkevich, V. A., Ivanova, O. E., Koroleva, G. A., Hinkkanen, A. E., Ilonen, J. (2003). Recombination in Circulating Enteroviruses. J. Virol. 77: 10423-10431 [Abstract] [Full Text]
Cherkasova, E., Laassri, M., Chizhikov, V., Korotkova, E., Dragunsky, E., Agol, V. I., Chumakov, K. (2003). Microarray analysis of evolution of RNA viruses: Evidence of circulation of virulent highly divergent vaccine-derived polioviruses. Proc. Natl. Acad. Sci. USA 100: 9398-9403 [Abstract] [Full Text]
Boot, H. J., Kasteel, D. T. J., Buisman, A.-M., Kimman, T. G. (2003). Excretion of Wild-Type and Vaccine-Derived Poliovirus in the Feces of Poliovirus Receptor-Transgenic Mice. J. Virol. 77: 6541-6545 [Abstract] [Full Text]
Lindberg, A. M., Andersson, P., Savolainen, C., Mulders, M. N., Hovi, T. (2003). Evolution of the genome of Human enterovirus B: incongruence between phylogenies of the VP1 and 3CD regions indicates frequent recombination within the species. J. Gen. Virol. 84: 1223-1235 [Abstract] [Full Text]
Blomqvist, S., Bruu, A.-L., Stenvik, M., Hovi, T. (2003). Characterization of a recombinant type 3/type 2 poliovirus isolated from a healthy vaccinee and containing a chimeric capsid protein VP1. J. Gen. Virol. 84: 573-580 [Abstract] [Full Text]
Martin, J., Samoilovich, E., Dunn, G., Lackenby, A., Feldman, E., Heath, A., Svirchevskaya, E., Cooper, G., Yermalovich, M., Minor, P. D. (2002). Isolation of an Intertypic Poliovirus Capsid Recombinant from a Child with Vaccine-Associated Paralytic Poliomyelitis. J. Virol. 76: 10921-10928 [Abstract] [Full Text]
Cherkasova, E. A., Korotkova, E. A., Yakovenko, M. L., Ivanova, O. E., Eremeeva, T. P., Chumakov, K. M., Agol, V. I. (2002). Long-Term Circulation of Vaccine-Derived Poliovirus That Causes Paralytic Disease. J. Virol. 76: 6791-6799 [Abstract] [Full Text]
Martin, J., Minor, P. D. (2002). Characterization of CHAT and Cox Type 1 Live-Attenuated Poliovirus Vaccine Strains. J. Virol. 76: 5339-5349 [Abstract] [Full Text]

J. Bacteriol.	Mol. Cell. Biol.	Microbiol. Mol. Biol. Rev.

Clin. Vaccine Immunol.	ALL ASM JOURNALS