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Journal of Virology, July 2001, p. 5740-5751, Vol. 75, No. 13
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.13.5740-5751.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Genomic Features of Intertypic Recombinant Sabin Poliovirus Strains Excreted by Primary Vaccinees

Nancy Stella Cuervo,1 Sophie Guillot,1 Natalia Romanenkova,2 Mariana Combiescu,3 André Aubert-Combiescu,3 Mohamed Seghier,4 Valérie Caro,1 Radu Crainic,1 and Francis Delpeyroux1,*

Epidémiologie Moléculaire des Entérovirus, Institut Pasteur, Paris, France1; Institut Pasteur de Saint-Pétersbourg, Saint Petersburg, Russia2; Institut Cantacuzino, Bucharest, Romania3; and Institut Pasteur d'Algérie, Algiers, Algeria4

Received 7 December 2000/Accepted 28 March 2001

The trivalent oral poliomyelitis vaccine (OPV) contains three different poliovirus serotypes. It use therefore creates particularly favorable conditions for mixed infection of gut cells, and indeed intertypic vaccine-derived recombinants (VdRec) have been frequently found in patients with vaccine-associated paralytic poliomyelitis. Nevertheless, there have not been extensive searches for VdRec in healthy vaccinees following immunization with OPV. To determine the incidence of VdRec and their excretion kinetics in primary vaccinees, and to establish the general genomic features of the corresponding recombinant genomes, we characterized poliovirus isolates excreted by vaccinees following primary immunization with OPV. Isolates were collected from 67 children 2 to 60 days following vaccination. Recombinant strains were identified by multiple restriction fragment length polymorphism assays. The localization of junction sites in recombinant genomes was also determined. VdRec excreted by vaccinees were first detected 2 to 4 days after vaccination. The highest rate of recombinants was on day 14. The frequency of VdRec depends strongly on the serotype of the analyzed isolates (2, 53, and 79% of recombinant strains in the last-excreted type 1, 2, and 3 isolates, respectively). Particular associations of genomic segments were preferred in the recombinant genomes, and recombination junctions were found in the genomic region encoding the nonstructural proteins. Recombination junctions generally clustered in particular subgenomic regions that were dependent on the serotype of the isolate and/or on the associations of genomic segments in recombinants. Thus, VdRec are frequently excreted by vaccinees, and the poliovirus replication machinery requirements or selection factors appear to act in vivo to shape the features of the recombinant genomes.

* Corresponding author. Mailing address: Laboratoire d'Epidémiologie Moléculaire des Entérovirus, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France. Phone: (33) 1 40 61 33 22. Fax: (33) 1 45 68 87 80. E-mail: delpeyro{at}pasteur.fr.

Journal of Virology, July 2001, p. 5740-5751, Vol. 75, No. 13
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.13.5740-5751.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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