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Journal of Virology, June 2001, p. 5697-5702, Vol. 75, No. 12
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.12.5697-5702.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Herpes Simplex Virus Nucleocapsids Mature to Progeny Virions by an Envelopment right-arrow  Deenvelopment right-arrow  Reenvelopment Pathway

J. N. Skepper,1 A. Whiteley,2,dagger H. Browne,2 and A. Minson2,*

Multi-Imaging Centre and Department of Anatomy1 and Division of Virology, Department of Pathology,2 University of Cambridge, Cambridge, United Kingdom

Received 4 December 2000/Accepted 26 March 2001

Herpes simplex virus (HSV) nucleocapsids acquire an envelope by budding through the inner nuclear membrane, but it is uncertain whether this envelope is retained during virus maturation and egress or whether mature progeny virions are derived by deenvelopment at the outer nuclear membrane followed by reenvelopment in a cytoplasmic compartment. To resolve this issue, we used immunogold electron microscopy to examine the distribution of glycoprotein D (gD) in cells infected with HSV-1 encoding a wild-type gD or a gD which is retrieved to the endoplasmic reticulum (ER). In cells infected with wild-type HSV-1, extracellular virions and virions in the perinuclear space bound approximately equal amounts of gD antibody. In cells infected with HSV-1 encoding an ER-retrieved gD, the inner and outer nuclear membranes were heavily gold labeled, as were perinuclear enveloped virions. Extracellular virions exhibited very little gold decoration (10- to 30-fold less than perinuclear virions). We conclude that the envelope of perinuclear virions must be lost during maturation and egress and that mature progeny virions must acquire an envelope from a post-ER cytoplasmic compartment. We noted also that gD appears to be excluded from the plasma membrane in cells infected with wild-type virus.

* Corresponding author. Mailing address: Department of Pathology, University of Cambridge, Tennis Court Rd., Cambridge CB2 1QP, United Kingdom. Phone: 44 1223-336920. Fax: 44 1223-336926. E-mail: acm{at}mole.bio.cam.ac.uk.

dagger Present address: Marie Curie Research Institute, The Chart, Oxted, Surrey RH9 0TL, United Kingdom.

Journal of Virology, June 2001, p. 5697-5702, Vol. 75, No. 12
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.12.5697-5702.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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