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Journal of Virology, June 2001, p. 5677-5683, Vol. 75, No. 12
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.12.5677-5683.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

The Adenovirus Type 5 E1B-55K Oncoprotein Actively Shuttles in Virus-Infected Cells, Whereas Transport of E4orf6 Is Mediated by a CRM1-Independent Mechanism

Tanja Dosch,1 Florian Horn,1 Grit Schneider,1 Friedrich Krätzer,1 Thomas Dobner,2 Joachim Hauber,1 and Roland H. Stauber1,*

Institute for Clinical and Molecular Virology, University of Erlangen-Nürnberg, D-91054 Erlangen,1 and Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, D-93053 Regensburg,2 Germany

Received 16 January 2001/Accepted 20 March 2001

The E1B-55K and E4orf6 proteins of adenovirus type 5 are involved in viral mRNA export. Here we demonstrate that adenovirus infection does not inhibit the function of the E1B-55K nuclear export signal and that E1B-55K also shuttles in infected cells. Even during virus infection, E1B-55K was exported by the leptomycin B-sensitive CRM1 pathway, whereas E4orf6 transport appeared to be mediated by an alternative mechanism. Our results strengthen the potential role of E1B-55K as the "driving force" for adenoviral late mRNA export.

* Corresponding author. Mailing address: Institute for Clinical and Molecular Virology, University of Erlangen-Nürnberg, Schlossgarten 4, D-91054 Erlangen, Germany. Phone: (49)-9131-8522102. Fax: (49)-9131-8522101. E-mail: rdstaube{at}viro.med.uni-erlangen.de.

Journal of Virology, June 2001, p. 5677-5683, Vol. 75, No. 12
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.12.5677-5683.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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