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Journal of Virology, June 2001, p. 5677-5683, Vol. 75, No. 12
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.12.5677-5683.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
The Adenovirus Type 5 E1B-55K Oncoprotein Actively
Shuttles in Virus-Infected Cells, Whereas Transport of E4orf6 Is
Mediated by a CRM1-Independent Mechanism
Tanja
Dosch,1
Florian
Horn,1
Grit
Schneider,1
Friedrich
Krätzer,1
Thomas
Dobner,2
Joachim
Hauber,1 and
Roland H.
Stauber1,*
Institute for Clinical and Molecular
Virology, University of Erlangen-Nürnberg, D-91054
Erlangen,1 and Institut für
Medizinische Mikrobiologie und Hygiene, Universität Regensburg,
D-93053 Regensburg,2 Germany
Received 16 January 2001/Accepted 20 March 2001
The E1B-55K and E4orf6 proteins of adenovirus type 5 are involved
in viral mRNA export. Here we demonstrate that adenovirus infection
does not inhibit the function of the E1B-55K nuclear export signal and
that E1B-55K also shuttles in infected cells. Even during virus
infection, E1B-55K was exported by the leptomycin B-sensitive CRM1
pathway, whereas E4orf6 transport appeared to be mediated by an
alternative mechanism. Our results strengthen the potential role of
E1B-55K as the "driving force" for adenoviral late mRNA export.
*
Corresponding author. Mailing address: Institute for
Clinical and Molecular Virology, University of Erlangen-Nürnberg,
Schlossgarten 4, D-91054 Erlangen, Germany. Phone: (49)-9131-8522102.
Fax: (49)-9131-8522101. E-mail:
rdstaube{at}viro.med.uni-erlangen.de.
Journal of Virology, June 2001, p. 5677-5683, Vol. 75, No. 12
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.12.5677-5683.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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