This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Neil, S.
Right arrow Articles by Collins, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Neil, S.
Right arrow Articles by Collins, M.

 Previous Article  |  Next Article 

Journal of Virology, June 2001, p. 5448-5456, Vol. 75, No. 12
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.12.5448-5456.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Postentry Restriction to Human Immunodeficiency Virus-Based Vector Transduction in Human Monocytes

Stuart Neil, Francisco Martin, Yasuhiro Ikeda, and Mary Collins*

Department of Immunology and Molecular Pathology, Windeyer Institute of Medical Sciences, University College London, London, United Kingdom

Received 6 December 2000/Accepted 8 March 2001

Cells of the monocyte lineage can be infected with human immunodeficiency virus type 1 (HIV-1) both during clinical infection and in vitro. The ability of HIV-1-based vectors to transduce human monocytes, monocyte-derived macrophages, and dendritic cells (DCs) was therefore examined, in order to develop an efficient protocol for antigen gene delivery to human antigen-presenting cells. Freshly isolated monocytes were refractory to HIV-1-based vector transduction but became transducible after in vitro differentiation to mature macrophages. This maturation-dependent transduction was independent of the HIV-1 accessory proteins Vif, Vpr, Vpu, and Nef in the packaging cells and of the central polypurine tract in the vector, and it was also observed with a vesicular stomatitis virus-pseudotyped HIV-1 provirus, defective only in envelope and Nef. The level and extent of reverse transcription of the HIV-1-based vector was similar after infection of immature monocytes and of mature macrophages. However, 2LTR vector circles could not be detected in monocytes, suggesting a block to vector nuclear entry in these cells. Transduction of freshly isolated monocytes exposed to HIV-1-based vector could be rescued by subsequent differentiation into DCs. This rescue was induced by fetal calf serum in the DC culture medium, which promoted vector nuclear entry.

* Corresponding author. Mailing address: Department of Immunology and Molecular Pathology, University College London, Windeyer Institute of Medical Sciences, 46 Cleveland St., London W1P 6DB, United Kingdom. Phone and fax: 44-207-679-9301. E-mail: mary.collins{at}ucl.ac.uk.

Journal of Virology, June 2001, p. 5448-5456, Vol. 75, No. 12
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.12.5448-5456.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Arfi, V., Lienard, J., Nguyen, X.-N., Berger, G., Rigal, D., Darlix, J.-L., Cimarelli, A. (2009). Characterization of the Behavior of Functional Viral Genomes during the Early Steps of Human Immunodeficiency Virus Type 1 Infection. J. Virol. 83: 7524-7535 [Abstract] [Full Text]  
  • Dong, C., Kwas, C., Wu, L. (2009). Transcriptional Restriction of Human Immunodeficiency Virus Type 1 Gene Expression in Undifferentiated Primary Monocytes. J. Virol. 83: 3518-3527 [Abstract] [Full Text]  
  • Rowe, H. M., Lopes, L., Brown, N., Efklidou, S., Smallie, T., Karrar, S., Kaye, P. M., Collins, M. K. (2009). Expression of vFLIP in a Lentiviral Vaccine Vector Activates NF-{kappa}B, Matures Dendritic Cells, and Increases CD8+ T-Cell Responses. J. Virol. 83: 1555-1562 [Abstract] [Full Text]  
  • Frecha, C., Costa, C., Negre, D., Gauthier, E., Russell, S. J., Cosset, F.-L., Verhoeyen, E. (2008). Stable transduction of quiescent T cells without induction of cycle progression by a novel lentiviral vector pseudotyped with measles virus glycoproteins. Blood 112: 4843-4852 [Abstract] [Full Text]  
  • Arfi, V., Riviere, L., Jarrosson-Wuilleme, L., Goujon, C., Rigal, D., Darlix, J.-L., Cimarelli, A. (2008). Characterization of the Early Steps of Infection of Primary Blood Monocytes by Human Immunodeficiency Virus Type 1. J. Virol. 82: 6557-6565 [Abstract] [Full Text]  
  • Munk, C., Zielonka, J., Constabel, H., Kloke, B.-P., Rengstl, B., Battenberg, M., Bonci, F., Pistello, M., Lochelt, M., Cichutek, K. (2007). Multiple Restrictions of Human Immunodeficiency Virus Type 1 in Feline Cells. J. Virol. 81: 7048-7060 [Abstract] [Full Text]  
  • Nitahara-Kasahara, Y., Kamata, M., Yamamoto, T., Zhang, X., Miyamoto, Y., Muneta, K., Iijima, S., Yoneda, Y., Tsunetsugu-Yokota, Y., Aida, Y. (2007). Novel Nuclear Import of Vpr Promoted by Importin {alpha} Is Crucial for Human Immunodeficiency Virus Type 1 Replication in Macrophages. J. Virol. 81: 5284-5293 [Abstract] [Full Text]  
  • Tsurutani, N., Yasuda, J., Yamamoto, N., Choi, B.-I., Kadoki, M., Iwakura, Y. (2007). Nuclear Import of the Preintegration Complex Is Blocked upon Infection by Human Immunodeficiency Virus Type 1 in Mouse Cells. J. Virol. 81: 677-688 [Abstract] [Full Text]  
  • Maheshwari, A., Smythies, L. E., Wu, X., Novak, L., Clements, R., Eckhoff, D., Lazenby, A. J., Britt, W. J., Smith, P. D. (2006). Cytomegalovirus blocks intestinal stroma-induced down-regulation of macrophage HIV-1 infection.. J. Leukoc. Biol. 80: 1111-1117 [Abstract] [Full Text]  
  • Cassol, E., Alfano, M., Biswas, P., Poli, G. (2006). Monocyte-derived macrophages and myeloid cell lines as targets of HIV-1 replication and persistence. J. Leukoc. Biol. 80: 1018-1030 [Abstract] [Full Text]  
  • Noser, J. A., Towers, G. J., Sakuma, R., Dumont, J.-M., Collins, M. K. L., Ikeda, Y. (2006). Cyclosporine increases human immunodeficiency virus type 1 vector transduction of primary mouse cells.. J. Virol. 80: 7769-7774 [Abstract] [Full Text]  
  • Chiu, Y.-L., Greene, W. C. (2006). APOBEC3 Cytidine Deaminases: Distinct Antiviral Actions along the Retroviral Life Cycle. J. Biol. Chem. 281: 8309-8312 [Abstract] [Full Text]  
  • Marchant, D., Neil, S. J. D., McKnight, A. (2006). Human immunodeficiency virus types 1 and 2 have different replication kinetics in human primary macrophage culture. J. Gen. Virol. 87: 411-418 [Abstract] [Full Text]  
  • Neil, S. J. D., McKnight, A., Gustafsson, K., Weiss, R. A. (2005). HIV-1 incorporates ABO histo-blood group antigens that sensitize virions to complement-mediated inactivation. Blood 105: 4693-4699 [Abstract] [Full Text]  
  • Arrighi, J.-F., Pion, M., Wiznerowicz, M., Geijtenbeek, T. B., Garcia, E., Abraham, S., Leuba, F., Dutoit, V., Ducrey-Rundquist, O., van Kooyk, Y., Trono, D., Piguet, V. (2004). Lentivirus-Mediated RNA Interference of DC-SIGN Expression Inhibits Human Immunodeficiency Virus Transmission from Dendritic Cells to T Cells. J. Virol. 78: 10848-10855 [Abstract] [Full Text]  
  • Triques, K., Stevenson, M. (2004). Characterization of Restrictions to Human Immunodeficiency Virus Type 1 Infection of Monocytes. J. Virol. 78: 5523-5527 [Abstract] [Full Text]  
  • Levy, D. N., Aldrovandi, G. M., Kutsch, O., Shaw, G. M. (2004). From The Cover: Dynamics of HIV-1 recombination in its natural target cells. Proc. Natl. Acad. Sci. USA 101: 4204-4209 [Abstract] [Full Text]  
  • Palmowski, M. J., Lopes, L., Ikeda, Y., Salio, M., Cerundolo, V., Collins, M. K. (2004). Intravenous Injection of a Lentiviral Vector Encoding NY-ESO-1 Induces an Effective CTL Response. J. Immunol. 172: 1582-1587 [Abstract] [Full Text]  
  • Mortlock, A., Low, W., Crisanti, A. (2003). Suppression of gene expression by a cell-permeable Tet repressor. Nucleic Acids Res 31: e152-e152 [Abstract] [Full Text]  
  • Field, N., Low, W., Daniels, M., Howell, S., Daviet, L., Boshoff, C., Collins, M. (2003). KSHV vFLIP binds to IKK-{gamma} to activate IKK. J. Cell Sci. 116: 3721-3728 [Abstract] [Full Text]  
  • Goujon, C., Jarrosson-Wuilleme, L., Bernaud, J., Rigal, D., Darlix, J.-L., Cimarelli, A. (2003). Heterologous Human Immunodeficiency Virus Type 1 Lentiviral Vectors Packaging a Simian Immunodeficiency Virus-Derived Genome Display a Specific Postentry Transduction Defect in Dendritic Cells. J. Virol. 77: 9295-9304 [Abstract] [Full Text]  
  • Fleury, S., Simeoni, E., Zuppinger, C., Deglon, N., von Segesser, L. K., Kappenberger, L., Vassalli, G. (2003). Multiply Attenuated, Self-Inactivating Lentiviral Vectors Efficiently Deliver and Express Genes for Extended Periods of Time in Adult Rat Cardiomyocytes In Vivo. Circulation 107: 2375-2382 [Abstract] [Full Text]  
  • Berthoux, L., Towers, G. J., Gurer, C., Salomoni, P., Pandolfi, P. P., Luban, J. (2003). As2O3 Enhances Retroviral Reverse Transcription and Counteracts Ref1 Antiviral Activity. J. Virol. 77: 3167-3180 [Abstract] [Full Text]  
  • Maurice, M., Verhoeyen, E., Salmon, P., Trono, D., Russell, S. J., Cosset, F.-L. (2002). Efficient gene transfer into human primary blood lymphocytes by surface-engineered lentiviral vectors that display a T cell-activating polypeptide. Blood 99: 2342-2350 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»