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Journal of Virology, June 2001, p. 5425-5428, Vol. 75, No. 11
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.11.5425-5428.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Determination of Essential Amino Acids Involved in the CD4-Independent Tropism of the X4 Human Immunodeficiency Virus Type 1 m7NDK Isolate: Role of Potential N Glycosylations in the C2 and V3 Regions of gp120

Julie Dumonceaux,1,dagger Caroline Goujon,1 Veronique Joliot,1,2,Dagger Pascale Briand,1 and Uriel Hazan1,2,*

INSERM Unite 380, Laboratoire de Génétique et Pathologie Expérimentales, Institut Cochin de Génétique Moléculaire, 75014 Paris,1 and Université Paris 7-Denis Diderot, UFR de Biochimie, 75005 Paris,2 France

Received 15 November 2000/Accepted 23 February 2001

Seven mutations in the C2, V3, and C3 regions of gp120 are implicated in the tropism of the first CD4-independent human immunodeficiency virus type 1 isolate, m7NDK. Site-directed mutagenesis revealed that three amino acids are essential to maintain this tropism, one in the C2 region and two in the V3 loop. Two mutations implied N glycosylation modifications.

* Corresponding author: Mailing address: INSERM Unité 380, Laboratoire de Pathologie et Genétique Expérimentales, Institut Cochin de Genétique Moléculaire, 22 Rue Méchain, 75014 Paris, France. Phone: 33-1-40516455. Fax: 33-1-40516407. E-mail: hazan{at}cochin.inserm.fr.

dagger Present address: Microbiology and Immunology Department, Albert Einstein College of Medicine, Bronx, NY 10461.

Dagger Present address: CNRS UMR146, Laboratoire de Régulations Cellulaires et Oncogénèse, Institut Curie, 91405 Orsay, France.

Journal of Virology, June 2001, p. 5425-5428, Vol. 75, No. 11
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.11.5425-5428.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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