Previous Article | Next Article ![]()
Journal of Virology, June 2001, p. 5421-5424, Vol. 75, No. 11
INSERM U544, Institut de Virologie, 67000 Strasbourg,1 and Maladies
Infectieuses, Hopital Cochin,2 and
Institut Pasteur,3 Paris, France
Received 19 October 2000/Accepted 23 February 2001
The factors present in serum and plasma samples of human
immunodeficiency virus (HIV)-infected patients that are responsible for
the neutralization of four HIV type 1 (HIV-1) primary isolates in vitro
have been analyzed. Purification of immunoglobulins (Ig) by affinity
chromatography showed that the activities were mostly attributable to
IgG and less frequently to IgA. For two samples, we have shown that the
high-level and broad-spectrum inhibitory activity was essentially
caused by non-Ig factors interfering with the measurement of
antibody-specific neutralizing activity.
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.11.5421-5424.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Immunoglobulin G (IgG) and IgA, but also
Nonantibody Factors, Account for In Vitro Neutralization of Human
Immunodeficiency Virus (HIV) Type 1 Primary Isolates by Serum and
Plasma of HIV-Infected Patients

*
Corresponding author. Mailing address: Institut de
Virologie, INSERM U544, 3 rue K
berlé, 67000 Strasbourg, France.
Phone: (33) 3 90 24 37 38. Fax: (33) 3 90 24 37 23. E-mail:
renaud.burrer{at}ulp.u-strasbg.fr.
Present address: Laboratoire de Spectrométrie, Ecole de
Chimie, Strasbourg, France.
This article has been cited by other articles:
| J. Bacteriol. | Mol. Cell. Biol. | Microbiol. Mol. Biol. Rev. |
|---|
| Clin. Vaccine Immunol. | ALL ASM JOURNALS |
|---|