This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An author's correction has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Burrer, R.
Right arrow Articles by Moog, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Burrer, R.
Right arrow Articles by Moog, C.

 Previous Article  |  Next Article 

Journal of Virology, June 2001, p. 5421-5424, Vol. 75, No. 11
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.11.5421-5424.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Immunoglobulin G (IgG) and IgA, but also Nonantibody Factors, Account for In Vitro Neutralization of Human Immunodeficiency Virus (HIV) Type 1 Primary Isolates by Serum and Plasma of HIV-Infected Patients

Renaud Burrer,1,* Dominique Salmon-Ceron,2 Sophie Richert,1,dagger Gianfranco Pancino,3 Gabriella Spiridon,2 Sandrine Haessig,1 Virginie Roques,1 Francoise Barre-Sinoussi,3 Anne-Marie Aubertin,1 and Christiane Moog1

INSERM U544, Institut de Virologie, 67000 Strasbourg,1 and Maladies Infectieuses, Hopital Cochin,2 and Institut Pasteur,3 Paris, France

Received 19 October 2000/Accepted 23 February 2001

The factors present in serum and plasma samples of human immunodeficiency virus (HIV)-infected patients that are responsible for the neutralization of four HIV type 1 (HIV-1) primary isolates in vitro have been analyzed. Purification of immunoglobulins (Ig) by affinity chromatography showed that the activities were mostly attributable to IgG and less frequently to IgA. For two samples, we have shown that the high-level and broad-spectrum inhibitory activity was essentially caused by non-Ig factors interfering with the measurement of antibody-specific neutralizing activity.

* Corresponding author. Mailing address: Institut de Virologie, INSERM U544, 3 rue Koeberlé, 67000 Strasbourg, France. Phone: (33) 3 90 24 37 38. Fax: (33) 3 90 24 37 23. E-mail: renaud.burrer{at}ulp.u-strasbg.fr.

dagger Present address: Laboratoire de Spectrométrie, Ecole de Chimie, Strasbourg, France.

Journal of Virology, June 2001, p. 5421-5424, Vol. 75, No. 11
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.11.5421-5424.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Harari, A., Bart, P.-A., Stohr, W., Tapia, G., Garcia, M., Medjitna-Rais, E., Burnet, S., Cellerai, C., Erlwein, O., Barber, T., Moog, C., Liljestrom, P., Wagner, R., Wolf, H., Kraehenbuhl, J.-P., Esteban, M., Heeney, J., Frachette, M.-J., Tartaglia, J., McCormack, S., Babiker, A., Weber, J., Pantaleo, G. (2008). An HIV-1 clade C DNA prime, NYVAC boost vaccine regimen induces reliable, polyfunctional, and long-lasting T cell responses. JEM 205: 63-77 [Abstract] [Full Text]  
  • Dhillon, A. K., Donners, H., Pantophlet, R., Johnson, W. E., Decker, J. M., Shaw, G. M., Lee, F.-H., Richman, D. D., Doms, R. W., Vanham, G., Burton, D. R. (2007). Dissecting the Neutralizing Antibody Specificities of Broadly Neutralizing Sera from Human Immunodeficiency Virus Type 1-Infected Donors. J. Virol. 81: 6548-6562 [Abstract] [Full Text]  
  • Sheppard, N. C., Davies, S. L., Jeffs, S. A., Vieira, S. M., Sattentau, Q. J. (2007). Production and Characterization of High-Affinity Human Monoclonal Antibodies to Human Immunodeficiency Virus Type 1 Envelope Glycoproteins in a Mouse Model Expressing Human Immunoglobulins. CVI 14: 157-167 [Abstract] [Full Text]  
  • Holl, V., Peressin, M., Schmidt, S., Decoville, T., Zolla-Pazner, S., Aubertin, A.-M., Moog, C. (2006). Efficient inhibition of HIV-1 replication in human immature monocyte-derived dendritic cells by purified anti-HIV-1 IgG without induction of maturation. Blood 107: 4466-4474 [Abstract] [Full Text]  
  • Holl, V., Hemmerter, S., Burrer, R., Schmidt, S., Bohbot, A., Aubertin, A.-M., Moog, C. (2004). Involvement of Fc{gamma}R I (CD64) in the Mechanism of HIV-1 Inhibition by Polyclonal IgG Purified from Infected Patients in Cultured Monocyte-Derived Macrophages. J. Immunol. 173: 6274-6283 [Abstract] [Full Text]  
  • Lorin, C., Mollet, L., Delebecque, F., Combredet, C., Hurtrel, B., Charneau, P., Brahic, M., Tangy, F. (2004). A Single Injection of Recombinant Measles Virus Vaccines Expressing Human Immunodeficiency Virus (HIV) Type 1 Clade B Envelope Glycoproteins Induces Neutralizing Antibodies and Cellular Immune Responses to HIV. J. Virol. 78: 146-157 [Abstract] [Full Text]  
  • Burrer, R., Haessig-Einius, S., Aubertin, A.-M., Moog, C. (2003). Polyclonal Immunoglobulin G from Patients Neutralizes Human Immunodeficiency Virus Type 1 Primary Isolates by Binding Free Virions, but without Interfering with an Initial CD4-Independent Attachment of the Virus to Primary Blood Mononuclear Cells. J. Virol. 77: 11385-11397 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»