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Journal of Virology, June 2001, p. 5421-5424, Vol. 75, No. 11
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.11.5421-5424.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Immunoglobulin G (IgG) and IgA, but also Nonantibody Factors, Account for In Vitro Neutralization of Human Immunodeficiency Virus (HIV) Type 1 Primary Isolates by Serum and Plasma of HIV-Infected Patients

Renaud Burrer,1,* Dominique Salmon-Ceron,2 Sophie Richert,1,dagger Gianfranco Pancino,3 Gabriella Spiridon,2 Sandrine Haessig,1 Virginie Roques,1 Francoise Barre-Sinoussi,3 Anne-Marie Aubertin,1 and Christiane Moog1

INSERM U544, Institut de Virologie, 67000 Strasbourg,1 and Maladies Infectieuses, Hopital Cochin,2 and Institut Pasteur,3 Paris, France

Received 19 October 2000/Accepted 23 February 2001

The factors present in serum and plasma samples of human immunodeficiency virus (HIV)-infected patients that are responsible for the neutralization of four HIV type 1 (HIV-1) primary isolates in vitro have been analyzed. Purification of immunoglobulins (Ig) by affinity chromatography showed that the activities were mostly attributable to IgG and less frequently to IgA. For two samples, we have shown that the high-level and broad-spectrum inhibitory activity was essentially caused by non-Ig factors interfering with the measurement of antibody-specific neutralizing activity.


* Corresponding author. Mailing address: Institut de Virologie, INSERM U544, 3 rue Koeberlé, 67000 Strasbourg, France. Phone: (33) 3 90 24 37 38. Fax: (33) 3 90 24 37 23. E-mail: renaud.burrer{at}ulp.u-strasbg.fr.

dagger Present address: Laboratoire de Spectrométrie, Ecole de Chimie, Strasbourg, France.


Journal of Virology, June 2001, p. 5421-5424, Vol. 75, No. 11
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.11.5421-5424.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.



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Copyright © 2001 by the American Society for Microbiology. All rights reserved.